Recombinant Anti-ICAD antibody [EPR4220] (ab108924)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4220] to ICAD
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ICAD antibody [EPR4220]
See all ICAD primary antibodies -
Description
Rabbit monoclonal [EPR4220] to ICAD -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat cell lysate, HeLa cell lysate; human breast carcinoma tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4220 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/250 - 1/500.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Inhibitor of the caspase-activated DNase (DFF40). -
Sequence similarities
Contains 1 CIDE-N domain. -
Post-translational
modificationsCaspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1676 Human
- Omim: 601882 Human
- SwissProt: O00273 Human
- Unigene: 484782 Human
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Alternative names
- A330085O09Rik antibody
- Caspase activated deoxyribonuclease inhibitor short form antibody
- DFF 1 antibody
see all
Images
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All lanes : Anti-ICAD antibody [EPR4220] (ab108924) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 37 kDa -
Immunohistochemical staining of paraffin-embedded human breast carcinoma using ab108924 at a diltion of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108924 has been referenced in 1 publication.
- Zhu CH et al. Investigation on the mechanisms of human sperm DNA damage based on the proteomics analysis by SWATH-MS. Clin Proteomics 20:2 (2023). PubMed: 36609216