Anti-ICAM1 antibody [MEM-111] (ab2213)
Key features and details
- Mouse monoclonal [MEM-111] to ICAM1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ICAM1 antibody [MEM-111]
See all ICAM1 primary antibodies -
Description
Mouse monoclonal [MEM-111] to ICAM1 -
Host species
Mouse -
Specificity
his antibody reacts with human CD54 antigen.
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Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human ICAM1. Burkitt's lymphoma cell line RAJI
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Positive control
- For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-111 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 1 - 2 µg/ml.
Use under non-reducing conditions. |
Flow Cyt | (3) |
Use a concentration of 0.5 - 4 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Use under non-reducing conditions. |
Flow Cyt
Use a concentration of 0.5 - 4 µg/ml. |
Target
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
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Alternative names
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
Images
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All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 2 µg/ml
Lane 1 : TF-1 cell lysates under non-reducing condition
Lane 2 : Raji cell lysates under non-reducing condition
Lane 3 : Jurkat cell lysates under non-reducing condition
Lane 4 : TF-1 cell lysates under reducing condition
Lane 5 : Raji cell lysates under reducing condition
Lane 6 : Jurkat cell lysates under reducing condition
Secondary
All lanes : IRDye800-conjugated anti-mouse secondary antibody
Observed band size: 88 kDa why is the actual band size different from the predicted? -
Flow cytometric analysis of Human Peripheral Blood cells labelling ICAM1 with ab2213 at 0.6 ug/ml showing separation of human monocytes (red-filled) from human lymphocytes (black-dashed).
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All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)
Lane 1 : TNF-alpha activated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : TNF-alpha nonactivated HUVEC cells
Performed under non-reducing conditions.
Observed band size: 90 kDa why is the actual band size different from the predicted?Lower bands represent tubulin as a loading control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (72)
ab2213 has been referenced in 72 publications.
- Harris AR et al. Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy. Front Oncol 12:801764 (2022). PubMed: 35372032
- Lim EJ et al. ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer. Cell Death Dis 13:417 (2022). PubMed: 35487888
- Sznurkowski JJ et al. Impact of Activation of EGFL7 within Microenvironment of High Grade Ovarian Serous Carcinoma on Infiltration of CD4+ and CD8+ Lymphocytes. Medicina (Kaunas) 58:N/A (2022). PubMed: 35630004
- Ding S et al. ICAM-1-related noncoding RNA accelerates atherosclerosis by amplifying NF-κB signaling. J Mol Cell Cardiol 170:75-86 (2022). PubMed: 35714558
- van den Braak WJP et al. Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines. Viruses 14:N/A (2022). PubMed: 36298792