Anti-IFIT3/P60 antibody [OTI1G1] (ab118045)
Key features and details
- Mouse monoclonal [OTI1G1] to IFIT3/P60
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-IFIT3/P60 antibody [OTI1G1]
See all IFIT3/P60 primary antibodies -
Description
Mouse monoclonal [OTI1G1] to IFIT3/P60 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Chimpanzee -
Immunogen
Recombinant full length protein corresponding to Human IFIT3/P60 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_001540).
Database link: O14879 -
Positive control
- WB: HepG2, HeLa, A549, COS-7, Jurkat and MCF7 extracts; HEK-293T cells transfected with pCMV6-ENTRY IFIT3/P60 cDNA. ICC/IF: COS-7 (transiently transfected by pCMV6-ENTRY IFIT3/P60 cDNA) and HT-29 cells. Flow Cyt (Intra): Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI1G1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 56 kDa.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/2000. Predicted molecular weight: 56 kDa. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Sequence similarities
Belongs to the IFIT family.
Contains 8 TPR repeats. - Information by UniProt
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Database links
- Entrez Gene: 738542 Chimpanzee
- Entrez Gene: 3437 Human
- Omim: 604650 Human
- SwissProt: A5A6J9 Chimpanzee
- SwissProt: O14879 Human
- Unigene: 47338 Human
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Alternative names
- CIG 49 antibody
- CIG49 antibody
- GARG 49 antibody
see all
Images
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All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 3 : SVT2 extracts
Lane 4 : A549 (Human lung carcinoma cell line) extracts
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 7 : MDCK (Canine kidney cell line) extracts
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) extracts
Lysates/proteins at 35 µg per lane.
Predicted band size: 56 kDa -
pCMV6-ENTRY IFIT3/P60 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
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ab118045 at 1/100 dilution staining IFIT3/P60 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
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HT-29 (Human colorectal adenocarcinoma cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
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All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells, transfected with pCMV6-ENTRY IFIT3/P60 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 56 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab118045 has not yet been referenced specifically in any publications.