Recombinant Anti-IGBP1 antibody [EPR12315] - BSA and Azide free (ab238932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12315] to IGBP1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IGBP1 antibody [EPR12315] - BSA and Azide free
See all IGBP1 primary antibodies -
Description
Rabbit monoclonal [EPR12315] to IGBP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human pancreas tissue.
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General notes
ab238932 is the carrier-free version of ab170911.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12315 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
Antigen retrieval is recommended. |
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ICC/IF |
Use at an assay dependent concentration.
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|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Antigen retrieval is recommended. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa. |
Target
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Function
Associated to surface IgM-receptor; may be involved in signal transduction. May be involved in regulation of the catalytic activity of type 2A-related serine/threonine phosphatases. -
Tissue specificity
Ubiquitously expressed with highest levels in heart, skeletal muscle and pancreas. -
Involvement in disease
Defects in IGBP1 are the cause of agenesis of the corpus callosum with mental retardation-ocular coloboma-micrognathia (ACCMRCM) [MIM:300472]. -
Sequence similarities
Belongs to the IGBP1/TAP42 family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3476 Human
- Entrez Gene: 18518 Mouse
- Entrez Gene: 58845 Rat
- Omim: 300139 Human
- SwissProt: P78318 Human
- SwissProt: Q61249 Mouse
- SwissProt: O08836 Rat
- Unigene: 496267 Human
see all -
Alternative names
- Alpha 4 antibody
- B cell signal transduction molecule alpha 4 antibody
- B-cell signal transduction molecule alpha 4 antibody
see all
Images
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Intracellular flow cytometric analysis of permeabilized K562 cells labeling IGBP1 using ab170911 at 1/100 dilution (red) or a rabbit IgG negative (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170911).
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IGBP1 using ab170911 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170911).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGBP1 using ab170911 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170911).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab238932 has not yet been referenced specifically in any publications.