Anti-IKZF3 antibody (ab88513)
Key features and details
- Mouse polyclonal to IKZF3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IKZF3 antibody
See all IKZF3 primary antibodies -
Description
Mouse polyclonal to IKZF3 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human IKZF3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Jurkat cell lysate, IK2F3 transfected 293T cell lysate IHC-P: Human tonsil tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
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IHC-P |
Use a concentration of 3 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
IHC-P
Use a concentration of 3 µg/ml. |
Target
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Function
Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner. -
Tissue specificity
Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. -
Sequence similarities
Belongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 22806 Human
- Omim: 606221 Human
- SwissProt: Q9UKT9 Human
- Unigene: 444388 Human
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Alternative names
- AIO antibody
- Aiolos antibody
- IKAROS family zinc finger 3 (Aiolos) antibody
see all
Images
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Anti-IKZF3 antibody (ab88513) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-IKZF3 antibody (ab88513) at 1 µg/ml
Lane 1 : IKZF3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
ab88513, at 3 µg/ml, staining IKZF3 in formalin fixed, paraffin embedded human tonsil tissue by Immunoperoxidase/Immunohistochemistry.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab88513 has not yet been referenced specifically in any publications.