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  1. Link

    il-1-beta-antibody-ab9722.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-1 beta antibody (ab9722)

  • Datasheet
Reviews (21)Q&A (13)References (591)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722)
  • Sandwich ELISA - Anti-IL-1 beta antibody (ab9722)
  • Western blot - Anti-IL-1 beta antibody (ab9722)

Key features and details

  • Rabbit polyclonal to IL-1 beta
  • Suitable for: IHC-P, Sandwich ELISA, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: unknown

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-IL-1 beta antibody [EPR21086] (ab216995)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-IL-1 beta antibody
    See all IL-1 beta primary antibodies
  • Description

    Rabbit polyclonal to IL-1 beta
  • Host species

    Rabbit
  • Specificity

    Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta.

  • Tested applications

    Suitable for: IHC-P, Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Full length protein aa 118-269. Full length mature protein minus the propeptide from aa 1-117 (Peptide available as ab9723).
    Database link: P10749

  • Positive control

    • IHC-P: Human adrenal tissue; Recombinant mouse IL-1 beta protein (ab9723) can be used as a positive control in WB
  • General notes

    This product is no longer batch tested in IHC, for an IHC validated antibody please see ab156791

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with sterile water to 0.1-1.0mg/ml, aliquot and store at -20°C.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    No preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    unknown
  • Light chain type

    unknown
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Obesity
    • Neuroscience
    • Processes

Associated products

  • Recombinant Protein

    • Recombinant human IL-1 beta protein (Active) (ab259387)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (5)
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml.

To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b.

WB (6)
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa.

Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Notes
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml.

To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b.

WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa.

Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function

    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • Tissue specificity

    Expressed in activated monocytes/macrophages (at protein level).
  • Sequence similarities

    Belongs to the IL-1 family.
  • Post-translational
    modifications

    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • Cellular localization

    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3553 Human
    • Omim: 147720 Human
    • SwissProt: P01584 Human
    • Unigene: 126256 Human
    • Alternative names

      • Catabolin antibody
      • H1 antibody
      • IFN beta inducing factor antibody
      • IL 1 antibody
      • IL 1 beta antibody
      • IL-1 beta antibody
      • IL1 antibody
      • IL1 BETA antibody
      • IL1B antibody
      • IL1B_HUMAN antibody
      • IL1F2 antibody
      • Interleukin 1 beta antibody
      • Interleukin 1 beta precursor antibody
      • interleukin 1, beta antibody
      • Interleukin-1 beta antibody
      • OAF antibody
      • Osteoclast activating factor antibody
      • OTTHUMP00000162031 antibody
      • Preinterleukin 1 beta antibody
      • Preinterleukin beta antibody
      • Pro interleukin 1 beta antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal adrenal tissue labelling IL-1 beta with ab9722 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

    • Sandwich ELISA - Anti-IL-1 beta antibody (ab9722)
      Sandwich ELISA - Anti-IL-1 beta antibody (ab9722)

      Sandwich ELISA detecting IL-1 beta using ab9722 at a concentration of 2 µg/ml.

    • Western blot - Anti-IL-1 beta antibody (ab9722)
      Western blot - Anti-IL-1 beta antibody (ab9722)

      To detect mouse IL-1 beta by Western Blot analysis, ab9722 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse IL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

      Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse IL-1 beta, respectively.

      Non-reducing conditions.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (591)

    Publishing research using ab9722? Please let us know so that we can cite the reference in this datasheet.

    ab9722 has been referenced in 591 publications.

    • Pang N  et al. Transcranial Ultrasound Stimulation of Hypothalamus in Aging Mice. IEEE Trans Ultrason Ferroelectr Freq Control 68:29-37 (2021). PubMed: 31985418
    • Lv Q  et al. Uric acid drives intestinal barrier dysfunction through TSPO-mediated NLRP3 inflammasome activation. Inflamm Res 70:127-137 (2021). PubMed: 33074353
    • Chang H  et al. Micro-ribonucleic acid-23a-3p prevents the onset of type 2 diabetes mellitus by suppressing the activation of nucleotide-binding oligomerization-like receptor family pyrin domain containing 3 inflammatory bodies-caused pyroptosis through negatively regulating NIMA-related kinase 7. J Diabetes Investig 12:334-345 (2021). PubMed: 32881354
    • Zhang H  et al. miR-34a-5p up-regulates the IL-1ß/COX2/PGE2 inflammation pathway and induces the release of CGRP via inhibition of SIRT1 in rat trigeminal ganglion neurons. FEBS Open Bio 11:300-311 (2021). PubMed: 33155431
    • Cheng W  et al. Paraventricular Nucleus P2X7 Receptors Aggravate Acute Myocardial Infarction Injury via ROS-Induced Vasopressin-V1b Activation in Rats. Neurosci Bull 37:641-656 (2021). PubMed: 33620697
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-IL-1 beta antibody

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (head and neck cancer tissue)
    Gel Running Conditions
    Reduced Denaturing (12% SDS page gel)
    Loading amount
    30 µg
    Specification
    head and neck cancer tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 2-8°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 18 2019

    Western blot abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse RPE)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Gel)
    Loading amount
    10 µg
    Treatment
    high fat diet, inflammation inhibitor, 6 months
    Specification
    mouse RPE
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 04 2019

    Western blot abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Treatment
    20 ug/ml poly(I:C)
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 17 2014

    Western blot abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (peritoneal macrophages)
    Gel Running Conditions
    Reduced Denaturing (10 % gel)
    Loading amount
    50 µg
    Treatment
    10 µg/ml LPS for 24hrs
    Specification
    peritoneal macrophages
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sandra Sobocanec

    Verified customer

    Submitted Mar 24 2010

    Western blot abreview for Anti-IL-1 beta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Alveolar macrophages (MHS cell line))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Treatment
    LPS (1ug/mL) 18 hours
    Specification
    Alveolar macrophages (MHS cell line)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 13 2008

    Western blot abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skin)
    Loading amount
    100 µg
    Specification
    skin
    Blocking step
    Other as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 50
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Precedia Massey

    Verified customer

    Submitted Apr 26 2006

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