Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-27-A
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with IL27-A.
  • Tested applications

    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human IL-27-A. 16 amino acid synthetic peptide near the amino terminus of human IL27-A.

  • Positive control

    • EL4 cell lysate and EL4 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 39, 51 kDa (predicted molecular weight: 27 kDa).
ICC Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation.
  • Tissue specificity

    Expressed in monocytes and in placenta.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Secreted. Does not seem to be secreted without coexpression of EBI3.
  • Information by UniProt
  • Database links

  • Alternative names

    • EBI3 antibody
    • IL 27 antibody
    • IL 27 p28 subunit antibody
    • IL 27A antibody
    • IL 30 antibody
    • IL-27 subunit alpha antibody
    • IL-27-A antibody
    • IL27 antibody
    • IL27-A antibody
    • IL27A antibody
    • IL27A_HUMAN antibody
    • IL27p28 antibody
    • IL30 antibody
    • Interleukin 27 antibody
    • Interleukin 27 subunit alpha antibody
    • Interleukin 27, 28-KD subunit antibody
    • Interleukin 30 antibody
    • Interleukin-27 subunit alpha antibody
    • p28 antibody
    see all

Images

  • Lane 1 : Anti-IL-27-A antibody (ab62501) at 1 µg/ml
    Lane 2 : Anti-IL-27-A antibody (ab62501) at 2 µg/ml

    All lanes : A20 (mouse reticulum sarcoma cell line) cell lysate

    Predicted band size: 27 kDa

  • Immunofluorescence analysis of Human dendritic cells, staining IL27-A with ab62501.

    Cells were fixed with methanol, permeabilized with 0.1% Saponin and blocked with 4% BSA for 30 minutes at 25&degC. Samples were incubated with primary antibody (10 &microg/ml in blocking buffer) for 14 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.

    See Abreview

References

ab62501 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (dendritic cell)
Permeabilization
Yes - 0,1% Saponin / PBS
Specification
dendritic cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Jan 02 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (PBMCs)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Loading amount
15 µg
Treatment
see below
Specification
PBMCs
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 04 2012

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