Anti-IL-8 antibody (ab7747)
Key features and details
- Rabbit polyclonal to IL-8
- Suitable for: ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-8 antibody
See all IL-8 primary antibodies -
Description
Rabbit polyclonal to IL-8 -
Host species
Rabbit -
Specificity
Please be aware that this product has low homology with the mouse and rat sequence of IL8 (Rat, 46%; Mouse 45%, UniProt blast) and we therefore cannot guarantee reactivity in these species. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to IL-8.
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Positive control
- ELISA: Human IL-8.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/50.
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Notes |
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ELISA
1/50. |
Target
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Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
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Specificity test of ab7747.
ab7747 was biotinylated and used as a detection antibody in different concentrations.
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Protocols
Datasheets and documents
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Datasheet download
References (65)
ab7747 has been referenced in 65 publications.
- Fitzpatrick CJ et al. Junin Virus Activates p38 MAPK and HSP27 Upon Entry. Front Cell Infect Microbiol 12:798978 (2022). PubMed: 35463647
- Tian Z et al. Targeted blocking of CCR2 and CXCR2 improves the efficacy of transarterial chemoembolization of hepatocarcinoma. Cancer Cell Int 22:362 (2022). PubMed: 36403057
- Abbasi M et al. Proteomic Analysis of Cardioembolic and Large Artery Atherosclerotic Clots Using Reverse Phase Protein Array Technology Reveals Key Cellular Interactions Within Clot Microenvironments. Cureus 13:e13499 (2021). PubMed: 33777584
- Cao R et al. Nell-1 attenuates lipopolysaccharide-induced inflammation in human dental pulp cells. J Mol Histol 52:671-680 (2021). PubMed: 33905072
- Wang Q et al. Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity. Bosn J Basic Med Sci 21:81-92 (2021). PubMed: 32343942