Recombinant Anti-IL-10 antibody [EPR1114] (ab133575)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1114] to IL-10
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-10 antibody [EPR1114]
See all IL-10 primary antibodies -
Description
Rabbit monoclonal [EPR1114] to IL-10 -
Host species
Rabbit -
Specificity
This antibody is not suitable for detection of endogenous expression of IL-10 in WB.
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Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- Flow Cyt (intra): HeLa cells; Human PBMCs. WB: Recombinant IL-10 protein. ICC/IF: Human peripheral blood mononuclear cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1114 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
1/50 - 1/250.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
This application will not work using endogenous samples. It reacts only with the recombinant fragment. |
Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50 - 1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa). This application will not work using endogenous samples. It reacts only with the recombinant fragment. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab133575 at a dilution of 1 in 300 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
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Unpurified ab133575 staining IL-10 in human peripheral blood mononuclear cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin in PBS and blocked with 4% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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Immunofluorescence staining of Molt-4 cells with purified ab133575 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab133575 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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Anti-IL-10 antibody [EPR1114] (ab133575) at 1/1000 dilution (unpurified) + Recombinant IL-10 protein at 0.005 µg
Secondary
Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of permeabilized HeLa cells labelling IL-10 using unpurified ab133575 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
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Intracellular flow cytometric analysis ofhuman PBMCs (peripheral blood mononuclear cells)treated with PHA labeling IL-10 with ab133575 at 1/100 dilution (green), followed by Goat anti-rabbit Alexa Fluorr® 488.
1 (dashed line) = isotype control, 2 (black line) = PBMCs untreated, 3 (green line) = PBMCs treated with PHA.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab133575 has been referenced in 12 publications.
- Shen P et al. Mechanical stress protects against chondrocyte pyroptosis through lipoxin A4 via synovial macrophage M2 subtype polarization in an osteoarthritis model. Biomed Pharmacother 153:113361 (2022). PubMed: 35797941
- Wang Q et al. Morphine suppresses the immune function of lung cancer by up-regulating MAEL expression. BMC Pharmacol Toxicol 23:92 (2022). PubMed: 36476246
- Chan L et al. Cytokine Profile in Plasma Extracellular Vesicles of Parkinson's Disease and the Association with Cognitive Function. Cells 10:N/A (2021). PubMed: 33803292
- Zhu H et al. Knockdown of RSAD2 attenuates B cell hyperactivity in patients with primary Sjögren's syndrome (pSS) via suppressing NF-?b signaling pathway. Mol Cell Biochem 476:2029-2037 (2021). PubMed: 33512636
- Zhang T et al. GPR18 Agonist Resolvin D2 Reduces Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage by Multiple Protective Mechanisms. Cell Mol Neurobiol N/A:N/A (2021). PubMed: 34089427