Anti-IL-17A antibody (ab79056)
Key features and details
- Rabbit polyclonal to IL-17A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IL-17A antibody
See all IL-17A primary antibodies -
Description
Rabbit polyclonal to IL-17A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
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Positive control
- IHC-P: lymphoid nodules
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General notes
The blocking peptide is available as ab190773
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 5 - 20 µg/ml.
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IHC-P | (9) |
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
Use a concentration of 0.125 - 0.5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).Can be blocked with IL-17A peptide (ab190773).
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Notes |
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.125 - 0.5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).Can be blocked with IL-17A peptide (ab190773). |
Target
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3605 Human
- Entrez Gene: 16171 Mouse
- Omim: 603149 Human
- SwissProt: Q16552 Human
- SwissProt: Q62386 Mouse
- Unigene: 41724 Human
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Alternative names
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Mouse Colon Tissue using ab79056 at 2 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of A-20 (mouse reticulum sarcoma cell line) cells labelling IL-17A with purified ab79056 at 5 µg/mL. Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (1/500) was used as the secondary antibody (green). Nuclei counterstained with DAPI (blue).
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Lane 1 : Anti-IL-17A antibody (ab79056) at 0.5 µg/ml
Lane 2 : Anti-IL-17A antibody (ab79056) at 1 µg/ml
All lanes : A-20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 22 kDa, 28 kDa, 32 kDa. We are unsure as to the identity of these extra bands.The higher molecular weight bands seen in the Western blot may represent glycosylated IL-17A.
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Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using ab79056 at 5 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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ab79056 staining IL-17A in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse thymus tissue staining IL-17A with ab79056 at 20µg/ml. Goat anti-rabbit IgG (1/500) was used as the secondary antibody (red).
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All lanes : Anti-IL-17A antibody (ab79056)
Lane 1 : human thymus tissue lysate at 1 µg/ml
Lane 2 : human thymus tissue lysate at 2 µg/ml
Predicted band size: 18 kDa -
Anti-IL-17A antibody (ab79056) at 1 µg/ml + Recombinant human IL-17 at 0.001 µg
Predicted band size: 18 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (142)
ab79056 has been referenced in 142 publications.
- Zhang ZT et al. Dexmedetomidine alleviates acute lung injury by promoting Tregs differentiation via activation of AMPK/SIRT1 pathway. Inflammopharmacology 31:423-438 (2023). PubMed: 36534240
- Wang Y et al. Xuanfei Baidu Decoction reduces acute lung injury by regulating infiltration of neutrophils and macrophages via PD-1/IL17A pathway. Pharmacol Res 176:106083 (2022). PubMed: 35033647
- Zeng P et al. Qing Zao Fang (QZF) Alleviates the Inflammatory Microenvironment of the Submandibular Gland in Sjögren's Syndrome Based on the PI3K/Akt/HIF-1α/VEGF Signaling Pathway. Dis Markers 2022:6153459 (2022). PubMed: 35140821
- Vitenberga-Verza Z et al. Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis. Pathogens 11:N/A (2022). PubMed: 35335696
- Zhang C et al. IL-17 exacerbates experimental autoimmune prostatitis via CXCL1/CXCL2-mediated neutrophil infiltration. Andrologia 54:e14455 (2022). PubMed: 35560069