Anti-IL-17A antibody (ab91649)
Key features and details
- Rabbit polyclonal to IL-17A
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IL-17A antibody
See all IL-17A primary antibodies -
Description
Rabbit polyclonal to IL-17A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Mouse IL-17 protein (ab50162) can be used as a positive control in WB. This antibody gave a positive signal in a mouse lymph node section.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16171 Mouse
- Entrez Gene: 301289 Rat
- SwissProt: Q62386 Mouse
- SwissProt: Q61453 Rat
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Alternative names
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
Images
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IHC image of IL-17A staining in mouse lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab91649 has been referenced in 10 publications.
- Azevedo MLV et al. Lung Neutrophilic Recruitment and IL-8/IL-17A Tissue Expression in COVID-19. Front Immunol 12:656350 (2021). PubMed: 33868301
- Lu L et al. V?4 T cell-derived IL-17A is essential for amplification of inflammatory cascades in ischemic brain tissue after stroke. Int Immunopharmacol 96:107678 (2021). PubMed: 34162129
- Choreño-Parra JA et al. Clinical and Immunological Factors That Distinguish COVID-19 From Pandemic Influenza A(H1N1). Front Immunol 12:593595 (2021). PubMed: 33995342
- Chen J et al. The Severity of CVB3-Induced Myocarditis Can Be Improved by Blocking the Orchestration of NLRP3 and Th17 in Balb/c Mice. Mediators Inflamm 2021:5551578 (2021). PubMed: 34093086
- Soley BDS et al. B1 and B2 kinin receptor blockade improves psoriasis-like disease. Br J Pharmacol N/A:N/A (2020). PubMed: 32335893
- Hsu PY et al. Cordycepin and a preparation fromCordyceps militarisinhibit malignant transformation and proliferation by decreasing EGFR and IL-17RA signaling in a murine oral cancer model. Oncotarget 8:93712-93728 (2017). PubMed: 29212184
- Cai S et al. IFN-? induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense. Mucosal Immunol 9:718-29 (2016). PubMed: 26349661
- Chou TF et al. Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1a for Th17 suppression. Nat Commun 7:11904 (2016). WB, IHC ; Human . PubMed: 27312851
- Patras KA et al. Characterization of host immunity during persistent vaginal colonization by Group B Streptococcus. Mucosal Immunol 8:1339-48 (2015). IHC . PubMed: 25850655
- Usategui A et al. A profibrotic role for thymic stromal lymphopoietin in systemic sclerosis. Ann Rheum Dis N/A:N/A (2013). IHC ; Mouse . PubMed: 23413283