APC Anti-IL-2 Receptor alpha antibody [3C7] (ab25485)
Key features and details
- APC Rat monoclonal [3C7] to IL-2 Receptor alpha
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2b
Overview
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Product name
APC Anti-IL-2 Receptor alpha antibody [3C7]
See all IL-2 Receptor alpha primary antibodies -
Description
APC Rat monoclonal [3C7] to IL-2 Receptor alpha -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
ab25485 recognises low affinity alpha chain IL-2 receptor (MW 55 kDa) (IL-2R alpha). -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to IL-2 Receptor alpha. IL-2 dependent BALB/c helper T cell clone HT2
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
3C7 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.3µg for 106 cells.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use 0.3µg for 106 cells. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16184 Mouse
- SwissProt: P01590 Mouse
- Unigene: 915 Mouse
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Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25485 has been referenced in 1 publication.
- Bursen A et al. The AF4.MLL fusion protein is capable of inducing ALL in mice without requirement of MLL.AF4. Blood 115:3570-9 (2010). Flow Cyt ; Mouse . PubMed: 20194896