Anti-IL-6 antibody [B-E8] (ab11449)
Key features and details
- Mouse monoclonal [B-E8] to IL-6
- Suitable for: IHC-Fr, ELISA, Neutralising
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IL-6 antibody [B-E8]
See all IL-6 primary antibodies -
Description
Mouse monoclonal [B-E8] to IL-6 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, ELISA, Neutralisingmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human IL-6.
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General notes
Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
B-E8 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration.
Max dilution: 20pg neutralizes activity of 1U of IL-6 |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. Max dilution: 20pg neutralizes activity of 1U of IL-6 |
Target
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
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Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab11449 has been referenced in 5 publications.
- Zhao D et al. Human neural stem cell tropism to metastatic breast cancer. Stem Cells 30:314-25 (2012). PubMed: 22084033
- Cejková J et al. Favorable effects of trehalose on the development of UVB-mediated antioxidant/pro-oxidant imbalance in the corneal epithelium, proinflammatory cytokine and matrix metalloproteinase induction, and heat shock protein 70 expression. Graefes Arch Clin Exp Ophthalmol 249:1185-94 (2011). IHC-Fr ; Rabbit . PubMed: 21494875
- Zenkel M et al. Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma. Am J Pathol 176:2868-79 (2010). IHC-Fr ; Human . PubMed: 20395431
- Kube D et al. STAT3 is constitutively activated in Hodgkin cell lines. Blood 98:762-70 (2001). Neutralising ; Human . PubMed: 11468177
- Klein B et al. Murine anti-interleukin-6 monoclonal antibody therapy for a patient with plasma cell leukemia. Blood 78:1198-204 (1991). Neutralising ; Human . PubMed: 1715218