Recombinant Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8365(B)] to IMPDH2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-IMPDH2 antibody [EPR8365(B)]
See all IMPDH2 primary antibodies -
Description
Rabbit monoclonal [EPR8365(B)] to IMPDH2 -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody shares 86% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human IMPDH2 aa 500-600 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa. K-562, MCF7 whole cell lysate, Rat and Mouse spleen lysate, DDDK tagged human full length IMPDH2 recombinant protein; IHC-P: Human colon and ovarian cancer tissue; ICC/IF: HeLa cells; Flow Cyt (Intra): K-562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.24 x 10 -9 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8365(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Notes |
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Flow Cyt (Intra)
1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. -
Tissue specificity
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. -
Pathway
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. -
Sequence similarities
Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
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Database links
- Entrez Gene: 3615 Human
- Entrez Gene: 23918 Mouse
- Entrez Gene: 301005 Rat
- Omim: 146691 Human
- SwissProt: P12268 Human
- SwissProt: P24547 Mouse
- SwissProt: E9PU28 Rat
- Unigene: 654400 Human
see all -
Alternative names
- IMDH2_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 2 antibody
- IMP dehydrogenase 2 antibody
see all
Images
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All lanes : Anti-IMPDH2 antibody [EPR8365(B)] (ab129165) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Rat spleen lysate
Lane 5 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 60 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST -
All lanes : Anti-IMPDH2 antibody [EPR8365(B)] (ab129165) at 1/1000 dilution
Lane 1 : DDDK tagged human full length IMPDH1 recombinant protein
Lane 2 : DDDK tagged human full length IMPDH2 recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 140 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling IMPDH2 with ab129165 at 1/4000 for 30 mins at room temperature. A Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Negative control using only secondary antibody. Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling IMPDH2 with ab129165 at 1/4000 for 30 mins at room temperature. A Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Negative control using only secondary antibody. Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labelling IMPDH2 (Green) with ab129165 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L, was used as the secondary antibody at a 1/1000. Tubulin (Red) was stained with ab195889, an Alexa Fluor® 594-conjugated anti-alpha tubulin antibody, at a 1/200 dilution. DAPI (Blue) was used as the nuclear counterstain. Negative control using only secondary antibody.
Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Intracellular Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling IMPDH2 with ab129165 at 1/20 dilution (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. Rabbit monoclonal - Alexa Fluor® 488 (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab129165 has been referenced in 5 publications.
- Calvo-Vidal MN et al. Oncogenic HSP90 Facilitates Metabolic Alterations in Aggressive B-cell Lymphomas. Cancer Res 81:5202-5216 (2021). PubMed: 34479963
- Bianchi-Smiraglia A et al. Regulation of local GTP availability controls RAC1 activity and cell invasion. Nat Commun 12:6091 (2021). PubMed: 34667203
- Duong-Ly KC et al. T cell activation triggers reversible inosine-5'-monophosphate dehydrogenase assembly. J Cell Sci 131:N/A (2018). PubMed: 30154209
- Anthony SA et al. Reconstituted IMPDH polymers accommodate both catalytically active and inactive conformations. Mol Biol Cell N/A:N/A (2017). WB ; Human . PubMed: 28794265
- Barfeld SJ et al. Myc-dependent purine biosynthesis affects nucleolar stress and therapy response in prostate cancer. Oncotarget 6:12587-602 (2015). IHC-P ; Human . PubMed: 25869206