Product nameAnti-ING2 antibody [EPR4597]
See all ING2 primary antibodies
DescriptionRabbit monoclonal [EPR4597] to ING2
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human ING2 aa 100-200. The exact sequence is proprietary.
- WB: T47-D, HepG2 or HT-1080 cell lysates IHC-P: Human kidney tissue This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 33 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
FunctionSeems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
Tissue specificityWidely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
Sequence similaritiesBelongs to the ING family.
Contains 1 PHD-type zinc finger.
DomainThe PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
Cellular localizationNucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
- Information by UniProt
- ING 1 like tumor suppressor protein antibody
- ING 1L antibody
- ING 2 antibody
All lanes : Anti-ING2 antibody [EPR4597] (ab109504) at 1/10000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
ICC/IF image of ab109504 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109504 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunohistochemical analysis of ING2 expression in formalin-fixed, paraffin-embedded Human kidney tissue using ab109504 at 1/1000 dilution.