Anti-IRS1 antibody (ab52167)
Key features and details
- Rabbit polyclonal to IRS1
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IRS1 antibody
See all IRS1 primary antibodies -
Description
Rabbit polyclonal to IRS1 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human IRS1. Synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-S-I-T).
Database link: P35568 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Predicted molecular weight: 132 kDa.
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IHC-P |
1/50 - 1/100.
Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 132 kDa. |
IHC-P
1/50 - 1/100. Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
Sequence similarities
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Post-translational
modificationsSerine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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Database links
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
see all -
Alternative names
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
Images
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All lanes : Anti-IRS1 antibody (ab52167) at 1/500 dilution
Lane 1 : A431 cell extract
Lane 2 : A431 cell extract with immunising peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 132 kDa
Observed band size: >175 kDa why is the actual band size different from the predicted? -
ab52167 at 1/50 dilution staining IRS1 in human breast carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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ICC/IF image of ab52167 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (56)
ab52167 has been referenced in 56 publications.
- Jiang A et al. Free Fatty Acid Impairs Myogenic Differentiation through the AMPKα-MicroRNA 206 Pathway. Mol Cell Biol 42:e0032721 (2022). PubMed: 34694913
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
- Zu Y et al. The roles of CPSF6 in proliferation, apoptosis and tumorigenicity of lung adenocarcinoma. Aging (Albany NY) 14:9300-9316 (2022). PubMed: 36446361
- Zhao T et al. The Effects of Erchen Decoction on Gut Microbiota and Lipid Metabolism Disorders in Zucker Diabetic Fatty Rats. Front Pharmacol 12:647529 (2021). PubMed: 34366839
- Bao S et al. Agriophyllum Oligosaccharides Ameliorate Diabetic Insulin Resistance Through INS-R/IRS/Glut4-Mediated Insulin Pathway in db/db Mice and MIN6 Cells. Front Pharmacol 12:656220 (2021). PubMed: 34497509