• Product name

    Anti-Insulin antibody [7F8]
    See all Insulin primary antibodies
  • Description

    Mouse monoclonal [7F8] to Insulin
  • Host species

  • Specificity

    Human insulin
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ELISAmore details
  • Species reactivity

    Reacts with: Cow, Human, Pig
  • Immunogen

    Full length native protein (purified) (Human).

  • General notes

    Kass for clone 7F8 is 7.5 x 109M-1




Our Abpromise guarantee covers the use of ab8302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Use at an assay dependent dilution.

IHC-Fr Use at an assay dependent concentration.

Use at an assay dependent dilution.




ELISA Use at an assay dependent concentration.

Use at an assay dependent dilution. For detection of insulin in two-site enzyme immunoassay, this antibody can be used in conjunction with ab8303 (D4B8) This antibody recognises a different epitope than the anti-proinsulin ab8301 (3A1).


  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all


  • Ab8302 staining human normal pancreas tissue. Staining is localised to extracellular spaces.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.


ab8302 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.
The antibodies you have chosen are absolutely fine. Please select any as per your requirements. These are all guaranteed for IHC-Fr which means if they don't work we will either be replacing them or will offer you refund.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Clin Chem. 1994 Apr;40(4):531-6. Related Articles, Links Different forms of insulin-like growth factor-binding protein-3 detected in serum and seminal plasma by immunofluorometric assay with monoclonal antibodies. Koistinen H, Seppala M, Koistinen R. Department I of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland. Monoclonal antibodies against recombinant insulin-like growth factor-binding protein-3 (IGFBP-3) were produced to study the various forms of IGFBP-3 and to develop a specific immunofluorometric assay. We tested seven antibodies that showed no cross-reactions with the other five human IGFBPs. By immunoblotting, the main bands in normal serum were seen at > 90, 45, 41, 29, and 25 kDa. In pregnancy serum, the 29-kDa was stronger, and the double band at 41-45 kDa was weaker or totally absent. We characterized two immunofluorometric assays. IGF-I had no effect on either assay. Added IGF-II lowered the amount of recombinant IGFBP-3 measured by the 5C11/7F8 assay, but not by the 1B6/5C11 assay. In normal serum and follicular fluid, IGFBP-3 measurements were lower by the 5C11/7F8 assay, but in most pregnancy sera and amniotic fluids the assays gave similar results. The 5C11/7F8 assay also detected IGFBP-3 in seminal plasma, whereas the 1B6/5C11 assay did not. We conclude that the results of IGFBP-3 measurement depend on the antibodies used, and that different antibodies are suitable for the IGFBP-3 measurement in different body fluids. PMID: 7511996 [PubMed - indexed for MEDLINE]

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