Overview

  • Product name

    Anti-Insulin antibody [EPR17359]
    See all Insulin primary antibodies
  • Description

    Rabbit monoclonal [EPR17359] to Insulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Insulin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01308

  • Positive control

    • IHC-P: Human, mouse and rat pancreas tissue. ICC/IF: BxPC-3 cells. IHC-FoFr: Mouse pancreas tissue. WB: Mouse pancreas lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
IHC-P 1/64000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.

Target

  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Images

  • Anti-Insulin antibody [EPR17359] (ab181547) at 1/1000 dilution + Mouse pancreas lysate 20 µg at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    Cytoplasm staining on islet cells of human pancreas is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling Insulin with ab181547 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green).

    Confocal image shows cytoplasmic staining on BxPC-3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: - ab181547 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

     

  • Immunohistochemical analysis of 4% paraformaldehyde perfusion fixed, frozen section of mouse pancreas tissue labeling Insulin with ab181547 at 1/1000 dilution, followed by Donkey anti-rabbit Alexa Fluor®594 at 1/1000 dilution. Cytoplasm staining on islet cells of mouse pancreas is observed. Counter stained with DAPI.

    Negative control: PBS instead of primary antibody; secondary antibody is Donkey anti-rabbit Alexa Fluor®594 at 1/1000 dilution.

  • Immunohistochemical analysis of formaldehyde fixed, frozen section of mouse pancreas tissue labeling Insulin with ab181547 at 1/2000 dilution, followed by Goat Alexa Fluor®488 at 1/1000 dilution. Permeabilised with 0.5% Triton X100.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of mouse pancreas is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of rat pancreas is observed. Counterstained with hematoxylin.

    Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon tissue with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    Negative staining on human colonic adenocarcinoma is observed. Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    Negative staining on human liver tissue is observed. Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Elbassuoni EA & Abdel Hafez SM Impact of chronic exercise on counteracting chronic stress-induced functional and morphological pancreatic changes in male albino rats. Cell Stress Chaperones 24:567-580 (2019). Read more (PubMed: 30903523) »
  • Barati G  et al. Differentiation of microfluidic-encapsulated trabecular meshwork mesenchymal stem cells into insulin producing cells and their impact on diabetic rats. J Cell Physiol 234:6801-6809 (2019). Read more (PubMed: 30317587) »
See all 11 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Pancreas)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Pancreas
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: 24°C
Fixative
Formaldehyde

Han-Byul Lee

Verified customer

Submitted May 30 2019

Application
Immunocytochemistry
Sample
Human Cultured Cells (Human)
Permeabilization
Yes - Triton-x
Specification
Human
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 22 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Zucker rat pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
Zucker rat pancreas
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 25 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 31 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 22 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 17 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA PH 9
Specification
Pancreas
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Formaldehyde

Dr. Toloo Taghian

Verified customer

Submitted Apr 22 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
Yes - 0.5% Triton X100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 31 2015

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