Overview

  • Product name
  • Description
    Rabbit polyclonal to Insulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cat, Dog, Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Insulin.

    Read Abcam's proprietary immunogen policy (Peptide available as ab86658.)

  • Positive control
    • This antibody gave a positive staining pattern in formalin-fixed paraffin-embedded Mouse Pancreas tissue section and Human pancreas tissue section. This antibody gave a positive result when used in the following methanol fixed cell lines: RIN-5F.

Properties

Applications

Our Abpromise guarantee covers the use of ab63820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease
    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the insulin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Images

  • ab63820 stained in RIN-5F cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63820 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab63820 staining Insulin in Mouse pancreas tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue section underwent formaldehyde fixation and enzymatic epitope retrieval step. The primary antibody was used at 1µg/ml and incubated with the sample for 1 hour. An HRP-conjugated Rabbit HRP polymer system was used undiluted with the secondary antibody.

  • IHC image of ab63820 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63820, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References

This product has been referenced in:
  • Kong S  et al. Kill two birds with one stone: making multi-transgenic pre-diabetes mouse models through insulin resistance and pancreatic apoptosis pathogenesis. PeerJ 6:e4542 (2018). Read more (PubMed: 29682407) »
  • Monazzam A  et al. Increased Expression of GLP-1R in Proliferating Islets of Men1 Mice is Detectable by [68Ga]Ga-DO3A-VS-Cys40-Exendin-4 /PET. Sci Rep 8:748 (2018). Read more (PubMed: 29335487) »
See all 17 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
ELISA
Sample
Cow Serum (Cow Serum (Testing antibody ab63820 for use in RIA)
Specification
Cow Serum (Testing antibody ab63820 for use in RIA
Type
Direct

Dr. Duane Keisler

Verified customer

Submitted Aug 22 2014

Answer

Thank you for your reply.

While we have a number of anti-Insulin antibodies which you would be able to conjugated to an organic fluorophore, we currently do not offer AlexaFluor 500 in our EasyLink conjugation kits. We do offer a number of other fluorophores including FITC which have similar excitation and emission wavelengths. More about our EasyLink conjugation kits and the fluorescent labels available may be found at:

https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

Although we have many anti-Insulin antibodies, when using a conjugation kit you will want to make sure the antibody which you will be conjugating to is purified and has a concentration of at least 0.5mg/ml. Two such products are ab46707 and ab63820.

https://www.abcam.com/insulin-antibody-in-05-azide-free-ab46707.html

https://www.abcam.com/insulin-antibody-in-05-azide-free-ab46707.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us. The antibody should recognize the B chain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your phone call.
I am checking with the lab regarding an ICC/IF image for ab124466 and will let you know when I hear back.
Below is the information about the testing discount program as well as your discount code:

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab63820 inICC/IF and human. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forICC/IF and human and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.

I have checked our insulin antibodies, but the majority was raised against the full length insulin protein. Only ab65454 was raised against the a peptide between 30˜90 amino acids and ab63820 which was raised against theimmunogen peptide residues overlaps with one of the regions mentioned.
ab65454 was tested in indirect ELISA, while ab63820 is not yet tested in ELISA.

PLease let me know if and which antibody you would like to try in zebrafish/ELISA, and I'd be happy to send you the discount code.

I look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Merci pour votre question.

Cet anticorps contient tous les anticorps du sérum de lapin qui ont une forte affinité contre l'immunogène. Nous n'avons pas testé l'identité des ces immunoglobulines. Ceci sont normalement les IgG. A ma connaissance, les IgG de lapin ne sont pas classifiés en isotypes.

J'espère que cet information vous est utile. N'hésitez pas à nous recontacter si vous avez des autres questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: autoclave
Permeabilization
Yes - tritonX
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2011

Answer

Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti Orexin ab35337, ab6214, ab16787, ab64565 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti leptin ab20101, ab20115, ab9749 and ab2095 Anti Insulin ab6995, ab63820, ab7760 and ab46707 Anti neuropeptide Y We ab30914, ab10341, ab6173, ab43871, ab53478. These antibodies are not yet tested in ELISA. Anti peptide YY  ab51537, ab51539 etc. Anti ACTH  ab20358, ab21003, ab104768,a b104619 Anti MCH ab37337; These are however not yet tested in ELISA. Please check the datasheets of these antibodies for more up-to-date information. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic

Abcam user community

Verified customer

Submitted May 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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