PE Anti-Integrin beta 4 antibody [346-11A] (ab216310)
Key features and details
- PE Rat monoclonal [346-11A] to Integrin beta 4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-Integrin beta 4 antibody [346-11A]
See all Integrin beta 4 primary antibodies -
Description
PE Rat monoclonal [346-11A] to Integrin beta 4 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Other Immunogen Type corresponding to Mouse Integrin beta 4. (Tumor-associated antigen TSP-180 from BALB/c lung carcinoma).
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Positive control
- Cell Line I.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab216310 was conjugated with Phycoerythrin under optimal conditions. The solution is free of unconjugated Phycoerythrin and unconjugated antibody. -
Clonality
Monoclonal -
Clone number
346-11A -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.25µg for 106 cells.
(in 100 µl volume). |
Notes |
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Flow Cyt
Use 0.25µg for 106 cells. (in 100 µl volume). |
Target
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Function
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
Involvement in disease
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
Domain
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 192897 Mouse
- SwissProt: A2A863 Mouse
- Unigene: 213873 Mouse
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Alternative names
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab216310 has been referenced in 1 publication.
- Yoshioka T et al. ß4 Integrin signaling induces expansion of prostate tumor progenitors. J Clin Invest 123:682-99 (2013). PubMed: 23348745