Biotin Anti-Integrin beta 4 antibody [439-9B] (ab95584)
Key features and details
- Biotin Rat monoclonal [439-9B] to Integrin beta 4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
Biotin Anti-Integrin beta 4 antibody [439-9B]
See all Integrin beta 4 primary antibodies -
Description
Biotin Rat monoclonal [439-9B] to Integrin beta 4 -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Integrin beta 4. Native protein
Database link: P16144 -
Positive control
- Flow Cyt: EDTA treated A549 cell line.
-
General notes
ab95584 has been reported to have no effect on adhesion of Human colon adenocarcinoma cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Primary antibody notes
ab95584 has been reported to have no effect on adhesion of Human colon adenocarcinoma cells -
Clonality
Monoclonal -
Clone number
439-9B -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.25µg for 105-8 cells.
ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
---|
Flow Cyt
Use 0.25µg for 105-8 cells. ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
Involvement in disease
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
Domain
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3691 Human
- Omim: 147557 Human
- SwissProt: P16144 Human
- Unigene: 632226 Human
-
Alternative names
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
Images
Datasheets and documents
-
Datasheet download
References (2)
ab95584 has been referenced in 2 publications.
- Simon-Assmann P et al. Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin. J Cell Sci 107 ( Pt 3):577-87 (1994). PubMed: 8006072
- Falcioni R et al. Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue. Cancer Res 48:816-21 (1988). PubMed: 2448027