Overview

  • Product name
    Anti-Integrin linked ILK antibody [EPR1592]
    See all Integrin linked ILK primary antibodies
  • Description
    Rabbit monoclonal [EPR1592] to Integrin linked ILK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Integrin linked ILK. The exact sequence is proprietary.

  • Positive control
    • HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899); HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR1592
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/500.

For unpurified use at 1/100 - 1/250

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
    • Tissue specificity
      Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 5 ANK repeats.
      Contains 1 protein kinase domain.
    • Domain
      A PH-like domain is involved in phosphatidylinositol phosphate binding.
    • Post-translational
      modifications
      Autophosphorylated on serine residues.
    • Cellular localization
      Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 59 kDa serine/threonine protein kinase antibody
      • 59 kDa serine/threonine-protein kinase antibody
      • DKFZp686F1765 antibody
      • Epididymis secretory protein Li 28 antibody
      • HEL S 28 antibody
      • ILK 1 antibody
      • ILK 2 antibody
      • ILK antibody
      • ILK-1 antibody
      • ILK-2 antibody
      • ILK_HUMAN antibody
      • ILK1 antibody
      • ILK2 antibody
      • Integrin linked kinase 2 antibody
      • Integrin linked Kinase antibody
      • Integrin linked protein kinase antibody
      • Integrin-linked protein kinase antibody
      • p59 antibody
      • p59ILK antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
      Lane 3: Hek293 cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76468 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab76468 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. ab76468 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labelling Integrin linked ILK (green) with purified ab76468 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.  

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Integrin linked ILK antibody [EPR1592] (ab76468) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 51 kDa
      Observed band size: 51 kDa

    • ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
    • ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
    • Overlay histogram showing HEK293 cells stained with ab76468 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76468, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References

    This product has been referenced in:
    • Choe SR  et al. RCP induces FAK phosphorylation and ovarian cancer cell invasion with inhibition by curcumin. Exp Mol Med 50:52 (2018). WB . Read more (PubMed: 29700289) »
    • Huang Y  et al. PD-1 blocks lytic granule polarization with concomitant impairment of integrin outside-in signaling in the natural killer cell immunological synapse. J Allergy Clin Immunol N/A:N/A (2018). Human . Read more (PubMed: 29679656) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (platelet)
    Loading amount
    25 µg
    Specification
    platelet
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jan 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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