Anti-KAT13C / NCOA2 antibody (ab10508)
Key features and details
- Rabbit polyclonal to KAT13C / NCOA2
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KAT13C / NCOA2 antibody
See all KAT13C / NCOA2 primary antibodies -
Description
Rabbit polyclonal to KAT13C / NCOA2 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to KAT13C/ NCOA2.
Database link: Q15596 -
Positive control
- Nuclear or cytosolic extract from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 1-4 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 159 kDa.
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Notes |
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IP
Use at 1-4 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 159 kDa. |
Target
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Function
Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. -
Involvement in disease
Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation. -
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
Domain
Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10499 Human
- Entrez Gene: 17978 Mouse
- Omim: 601993 Human
- SwissProt: Q15596 Human
- SwissProt: Q61026 Mouse
- Unigene: 446678 Human
- Unigene: 2537 Mouse
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Alternative names
- bHLHe75 antibody
- Class E basic helix-loop-helix protein 75 antibody
- glucocorticoid receptor interacting protein 1 antibody
see all
Images
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All lanes : Anti-KAT13C / NCOA2 antibody (ab10508) at 1 µg/ml
Lane 1 : TCMK-1 whole cell lysate
Lane 2 : 4T1 whole cell lysate
Lane 3 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 159 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling KAT13C / NCOA2 with ab10508 at 1/1000 (1µg/ml). Detection: DAB.
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ab10508 immunoprecipitation of human NCOA2 (SRC2). Samples: Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: ab10508 used at 20
µ g/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab10508 has been referenced in 1 publication.
- Zhang Y et al. A systems biology-based classifier for hepatocellular carcinoma diagnosis. PLoS One 6:e22426 (2011). IHC-P ; Human . PubMed: 21829460