Anti-KAT13D / CLOCK antibody (ab178525)
Key features and details
- Goat polyclonal to KAT13D / CLOCK
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-KAT13D / CLOCK antibody
See all KAT13D / CLOCK primary antibodies -
Description
Goat polyclonal to KAT13D / CLOCK -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Chicken, Human, Orangutan -
Immunogen
Synthetic peptide corresponding to Human KAT13D/ CLOCK aa 458-472 (internal sequence) (Cysteine residue). (NP_004889.1)
Sequence:DTSTPPRQHLPAHEK
Database link: O15516 -
Positive control
- Mouse and rat skeletal lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab178525 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 95 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 95 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL. -
Tissue specificity
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsPhosphorylation is dependent on CLOCK-ARNTL heterodimer formation. -
Cellular localization
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 373991 Chicken
- Entrez Gene: 9575 Human
- Entrez Gene: 12753 Mouse
- Entrez Gene: 100172630 Orangutan
- Entrez Gene: 60447 Rat
- Omim: 601851 Human
- SwissProt: Q8QGQ6 Chicken
- SwissProt: O15516 Human
see all -
Alternative names
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab178525 has not yet been referenced specifically in any publications.