• Product name
    Anti-KDM5C / Jarid1C / SMCX antibody
    See all KDM5C / Jarid1C / SMCX primary antibodies
  • Description
    Rabbit polyclonal to KDM5C / Jarid1C / SMCX
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human KDM5C/ Jarid1C/ SMCX aa 275-325.
    Database link: P41229

  • Positive control
    • HeLa whole cell lysate.



Our Abpromise guarantee covers the use of ab72152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa).
IP Use at 2 µg/mg of lysate.


  • Function
    Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
  • Tissue specificity
    Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.
  • Involvement in disease
    Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
  • Sequence similarities
    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
  • Domain
    The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DXS1272E antibody
    • Histone demethylase JARID1C antibody
    • JARID1C antibody
    • JmjC domain containing protein SMCX antibody
    • Jumonji AT rich interactive domain 1C antibody
    • Jumonji, AT rich interactive domain 1C (RBP2 like) antibody
    • Jumonji/ARID domain-containing protein 1C antibody
    • KDM5C antibody
    • KDM5C_HUMAN antibody
    • Lysine (K) specific demethylase 5C antibody
    • Lysine-specific demethylase 5C antibody
    • MRX13 antibody
    • MRXJ antibody
    • MRXSCJ antibody
    • MRXSJ antibody
    • Protein SmcX antibody
    • Protein Xe169 antibody
    • rbp2 like protein antibody
    • Selected cDNA on X antibody
    • SMCX antibody
    • Smcx homolog X chromosome antibody
    • SmcX protein antibody
    • Smcy homolog X linked antibody
    • XE169 antibody
    • Xe169 protein antibody
    see all


  • All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab72152) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 176 kDa
    Observed band size: 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 220 kDa, 300 kDa. We are unsure as to the identity of these extra bands.

  • Detection of Human KDM5C / Jarid1C / SMCX by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab72152 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.


This product has been referenced in:
  • Liang Z  et al. Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin. Epigenetics Chromatin 4:18 (2011). Read more (PubMed: 21996408) »

See 1 Publication for this product

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