Anti-KIF5B antibody (ab25715)
Key features and details
- Rabbit polyclonal to KIF5B
- Suitable for: WB
- Reacts with: Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-KIF5B antibody
See all KIF5B primary antibodies -
Description
Rabbit polyclonal to KIF5B -
Host species
Rabbit -
Specificity
This antibody detects total level of endogenous kinesin protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment corresponding to Human KIF5B. Stalk domain of human KIF5B (amino acids 331-906) expressed in E. coli (FKHC3).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from rabbit serum by afinity chromatography. > 95% pure (by SDS PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Predicted molecular weight: 110 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 110 kDa. |
Target
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Function
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. -
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
Contains 1 kinesin motor domain. -
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
Cellular localization
Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons. - Information by UniProt
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Database links
- Entrez Gene: 3799 Human
- Omim: 602809 Human
- SwissProt: P33176 Human
- Unigene: 327736 Human
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Alternative names
- Conventional kinesin heavy chain antibody
- KIF 5B antibody
- KIF5B antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab25715 has been referenced in 4 publications.
- Furey C et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
- Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). PubMed: 28930676
- Malikov V et al. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nat Commun 6:6660 (2015). WB, ICC/IF . PubMed: 25818806
- Chu Y et al. Alterations in axonal transport motor proteins in sporadic and experimental Parkinson's disease. Brain 135:2058-73 (2012). ICC/IF ; Rat . PubMed: 22719003