Anti-KLRG1 antibody [2F1] (ab25054)
Key features and details
- Syrian hamster monoclonal [2F1] to KLRG1
- Suitable for: IP, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-KLRG1 antibody [2F1]
See all KLRG1 primary antibodies -
Description
Syrian hamster monoclonal [2F1] to KLRG1 -
Host species
Syrian hamster -
Tested applications
Suitable for: IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse KLRG1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.88% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
2F1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells. -
Tissue specificity
Expressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. Upon phosphorylation of ITIM motif KLRG1 associates with the two phosphatases PTPN11 and INPP5D. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 50928 Mouse
- SwissProt: O88713 Mouse
- Unigene: 20434 Mouse
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Alternative names
- 2F1 Ag antibody
- 2F1 antibody
- C type lectin domain family 15 member A antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab25054 has been referenced in 3 publications.
- Bouchentouf M et al. Induction of cardiac angiogenesis requires killer cell lectin-like receptor 1 and a4ß7 integrin expression by NK cells. J Immunol 185:7014-25 (2010). PubMed: 20971926
- Corral L et al. NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules. Eur J Immunol 30:920-30 (2000). PubMed: 10741410
- Hanke T et al. 2F1 antigen, the mouse homolog of the rat "mast cell function-associated antigen", is a lectin-like type II transmembrane receptor expressed by natural killer cells. Eur J Immunol 28:4409-17 (1998). PubMed: 9862378