Anti-KPNA2 antibody (ab84440)
Key features and details
- Rabbit polyclonal to KPNA2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KPNA2 antibody
See all KPNA2 primary antibodies -
Description
Rabbit polyclonal to KPNA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Orangutan -
Immunogen
Synthetic peptide corresponding to a region within C terminal amino acids 475-529 (ALQNHENESV YKASLSLIEK YFSVEEEEDQ NVVPETTSEG YTFQVQDGAP GTFNF) of human KPNA2 (NP_002257.1).
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General notes
Concentration is optimized for IHC and not determined
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
Target
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Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
Domain
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3838 Human
- Entrez Gene: 16647 Mouse
- Omim: 600685 Human
- SwissProt: P52292 Human
- SwissProt: P52293 Mouse
- Unigene: 594238 Human
- Unigene: 12508 Mouse
- Unigene: 391577 Mouse
see all -
Alternative names
- IMA1_HUMAN antibody
- Importin alpha 1 antibody
- Importin alpha 2 antibody
see all
Images
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Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human metastatic lymph node tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human bladder carcinoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse squamous cell carcinoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse teratoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of Human ovarian germ cell tumour, staining KPNA2 with ab84440.
Antigen retrieval was performed by submerging the sample in citrate buffer (pH 6) and microwaving. The sections were blocked with 1% BSA before incubating with primary antibody (1/400). Staining was detected using DAB.
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab84440 has been referenced in 26 publications.
- Miyamoto Y et al. SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication. Commun Biol 5:483 (2022). PubMed: 35590097
- Dong L et al. MiR-101-3p targets KPNA2 to inhibit the progression of lung squamous cell carcinoma cell lines. Histol Histopathol N/A:18573 (2022). PubMed: 36583484
- Zhou KX et al. Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC. J Immunol Res 2021:6694392 (2021). PubMed: 33728352
- Zeng F et al. KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. J Transl Med 19:112 (2021). PubMed: 33731128
- Fan X et al. Silencing of KPNA2 inhibits high glucose-induced podocyte injury via inactivation of mTORC1/p70S6K signaling pathway. Biochem Biophys Res Commun 521:1017-1023 (2020). PubMed: 31727365