Anti-KRT81 antibody [3B10-5B10] (ab55407)
Key features and details
- Mouse monoclonal [3B10-5B10] to KRT81
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-KRT81 antibody [3B10-5B10]
See all KRT81 primary antibodies -
Description
Mouse monoclonal [3B10-5B10] to KRT81 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 304-505 of Human KRT81.
-
General notes
This product was changed from ascites to tissue culture supernatant on 27/June/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
3B10-5B10 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | ||
ICC/IF | ||
IHC-P |
Notes |
---|
IF/ICC: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Tissue specificity
Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue. -
Involvement in disease
Defects in KRT81 are a cause of monilethrix (MLTRX) [MIM:158000]. Monilethrix is an autosomal dominant hair disorder characterized clinically by alopecia and follicular papules. Affected hairs have uniform elliptical nodes of normal thickness and intermittent constrictions, internodes at which the hair easily breaks. Usually only the scalp is involved, but in severe forms, the secondary sexual hair, eyebrows, eyelashes, and nails may also be affected. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
-
Database links
- Entrez Gene: 3887 Human
- Omim: 602153 Human
- SwissProt: Q14533 Human
- Unigene: 658118 Human
-
Alternative names
- 1 antibody
- basic antibody
- ghHb 1 antibody
see all
Images
-
KRT81 antibody (ab55407) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus. This was producted using the ascites version of the product.
-
KRT81 antibody (ab55407) used in immunofluorescence at 10ug/ml on HeLa cells. This was producted using the ascites version of the product.
-
KRT81 antibody (ab55407) at 1ug/lane + HepG2 cell lysate at 25ug/lane. This was producted using the ascites version of the product.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab55407 has been referenced in 2 publications.
- Wang X et al. Single-cell RNA-seq reveals the genesis and heterogeneity of tumor microenvironment in pancreatic undifferentiated carcinoma with osteoclast-like giant-cells. Mol Cancer 21:133 (2022). PubMed: 35733218
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873