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Enzymatic triple-staining IHC kits

Related

  • All IHC kits and reagents
    • Blocking reagents
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            • IHC-P protocol
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                Simplifying multicolor enzymatic IHC.

                TripleStain IHC detection systems

                TripleStain enzymatic IHC detection systems provide you with reagents and optimized protocols for staining three antigens on a tissue-section using immuno-enzymatic detection.

                • Animal specific kits have been tested of paraffin embedded mouse tissue
                • Human specific kits have been tested on paraffin embedded human tissue

                The kits also allow various combinations of species primary antibodies and a choice enzymatic labels (please use the key below to identify the most suitable system).

                These multicolor enzymatic systems offer

                • Reduced background
                  • when staining tissue containing endogenous biotin (eg kidney, liver and spleen) using an HRP and AP polymer based system
                • Optimized protocols
                  • staining in five hours excluding antigen retrieval steps
                • Various color combinations
                  • brown, red, green, brown and black depending on kit
                • Can be permanently mounted with a coverslip
                • Use  of mouse antibodies on mouse tissue
                  • protocols for blocking endogenous mouse IgG

                Example staining

                Hu-Colon-Cancer-RxMxM

                Staining of FFPE Human colon cancer tissue section using a Rabbit antibody against CEA (brown), a Mouse antibody against PCNA (black) and a Mouse antibody against smooth muscle actin (red) using  TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & DAB/Ni).

                Kit selection key

                Primary antibody keyChromogen key
                G: Goat, M: Mouse, R: Rabbit, Rt: RatDAB: Brown, AP/Red & FastRed: Red, HRP/Green: Green, DAB/Ni: Black

                Size guidelines if using 100µl polymer conjugate per slide : 24 mL (~120 slides), 72 mL (~360 slides), 240 mL (1200 slides).


                Human tissue staining:

                Product nameSize
                Product code
                TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & HRP/Green)24 mL, 72 mL, 240 mL
                ab183286
                TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & DAB/Ni)24 mL, 72 mL, 240 mL
                ab183287
                TripleStain IHC kit: R&R&M on human tissue (DAB, AP/Red & HRP/Green)24 mL, 72 mL, 240 mL
                ab183288
                TripleStain IHC kit: R&R&M on human tissue (DAB, AP/Red & DAB/Ni)24 mL, 72 mL, 240 mL
                ab183289
                TripleStain IHC kit: M&R&G on human tissue (DAB, AP/Red & HRP/Green)24 mL, 72 mL, 240 mL
                ab183290
                TripleStain IHC kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni)24 mL, 72 mL, 240 mL
                ab183291


                Rodent tissue staining

                Product nameSizeProduct code
                TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red)24 mL, 72 mL, 240 mL
                ab183292
                TripleStain IHC kit: M&M&R on rodent tissue (DAB, AP/Red & DAB/Ni)24 mL, 72 mL, 240 mL
                ab183294
                TripleStain IHC kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green)24 mL, 72 mL, 240 mL
                ab183295
                TripleStain IHC kit: R&R&M on rodent tissue (DAB, AP/Red & DAB/Ni)24 mL, 72 mL, 240 mL
                ab183296
                TripleStain IHC kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red)24 mL, 72 mL, 240 mL
                ab183297
                TripleStain IHC kit: M&R&Rt on rodent tissue (DAB, DAB/Ni & AP/Red)24 mL, 72 mL, 240 mL
                ab183298


                Applications

                Our triple-staining IHC kits can enable you to perform multicolor IHC experiments where you can

                • Stain different cell types to get an overview of their location in tissue specimens
                • Identify cells using a structural marker as well as an antibody against your target protein
                • Search for co-localization when combining cellular markers with activation markers
                • Perform histology experiments using visible color with no need to use a fluorescence microscope
                • Combine with fluorescent in-situ hybridization markers to simultaneously study protein and mRNA protein levels
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