Enzymatic triple-staining IHC kits
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Simplifying multicolor enzymatic IHC
TripleStain IHC Detection Systems
TripleStain enzymatic IHC detection systems provide you with reagents and optimized protocols for staining three antigens on a tissue-section using immuno-enzymatic detection.
- Animal specific kits have been tested of paraffin embedded mouse tissue
- Human specific kits have been tested on paraffin embedded human tissue
The kits also allow various combinations of species primary antibodies and a choice enzymatic labels (please use the key below to identify the most suitable system).
These multicolor enzymatic systems offer:
- Reduced background
- when staining tissue containing endogenous biotin (e.g. kidney, liver & spleen) using an HRP and AP polymer based system
- Optimized protocols
- staining in five hours excluding antigen retrieval steps
- Various color combinations
- brown, red, green, brown & black depending on kit
- Can be permanently mounted with a coverslip
- Use of mouse antibodies on mouse tissue
- protocols for blocking endogenous mouse IgG
Example staining
Staining of FFPE Human colon cancer tissue section using a Rabbit antibody against CEA (brown), a Mouse antibody against PCNA (black) and a Mouse antibody against smooth muscle actin (red) using TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & DAB/Ni).
Kit selection key:
| Primary antibody key | Chromogen key |
| G: Goat, M: Mouse, R: Rabbit, Rt: Rat | DAB: Brown, AP/Red & FastRed: Red, HRP/Green: Green, DAB/Ni: Black |
Size guidelines if using 100µl polymer conjugate per slide : 24ml (~120 slides), 72ml (~360 slides), 240ml (1200 slides).
Human Tissue Staining:
| Product name | Size | Product code |
| TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & HRP/Green) | 24ml, 72ml, 240ml | ab183286 |
| TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & DAB/Ni) | 24ml, 72ml, 240ml | ab183287 |
| TripleStain IHC kit: R&R&M on human tissue (DAB, AP/Red & HRP/Green) | 24ml, 72ml, 240ml | ab183288 |
| TripleStain IHC kit: R&R&M on human tissue (DAB, AP/Red & DAB/Ni) | 24ml, 72ml, 240ml | ab183289 |
| TripleStain IHC kit: M&R&G on human tissue (DAB, AP/Red & HRP/Green) | 24ml, 72ml, 240ml | ab183290 |
| TripleStain IHC kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni) | 24ml, 72ml, 240ml | ab183291 |
Rodent Tissue Staining:
| Product name | Size | Product code |
| TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red) | 24ml, 72ml, 240ml | ab183292 |
| TripleStain IHC kit: M&M&R on rodent tissue (DAB, AP/Red & DAB/Ni) | 24ml, 72ml, 240ml | ab183294 |
| TripleStain IHC kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green) | 24ml, 72ml, 240ml | ab183295 |
| TripleStain IHC kit: R&R&M on rodent tissue (DAB, AP/Red & DAB/Ni) | 24ml, 72ml, 240ml | ab183296 |
| TripleStain IHC kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red) | 24ml, 72ml, 240ml | ab183297 |
| TripleStain IHC kit: M&R&Rt on rodent tissue (DAB, DAB/Ni & AP/Red) | 24ml, 72ml, 240ml | ab183298 |
Applications
Our double-staining IHC kits can enable you to perform multicolor IHC experiments where you can:
- Stain different cell types to get an overview of their location in tissue specimens
- Identify cells using a structural marker as well as an antibody against your target protein
- Search for co-localization when combining cellular markers with activation markers
- Perform histology experiments using visible color with no need to use a fluorescence microscope
- Combine with fluorescent in-situ hybridization markers to simultaneously study protein and mRNA protein levels