Recombinant Anti-Ku80 antibody [EPR3468] (ab80592)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3468] to Ku80
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ku80 antibody [EPR3468]
See all Ku80 primary antibodies -
Description
Rabbit monoclonal [EPR3468] to Ku80 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: lysates from A549, HeLa, HepG2 and MCF7 cells; IHC-P: Human tonsil tissue; ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3468 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
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IP |
1/50.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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ICC/IF |
1/500 - 1/1000.
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Notes |
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Flow Cyt (Intra)
1/20. |
WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa. |
IP
1/50. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. -
Sequence similarities
Belongs to the ku80 family.
Contains 1 Ku domain. -
Developmental stage
Expression increases during promyelocyte differentiation. -
Domain
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. -
Post-translational
modificationsPhosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
Sumoylated. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 7520 Human
- Entrez Gene: 22596 Mouse
- Entrez Gene: 363247 Rat
- Omim: 194364 Human
- SwissProt: P13010 Human
- SwissProt: P27641 Mouse
- Unigene: 388739 Human
- Unigene: 246952 Mouse
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Alternative names
- 86 kDa subunit of Ku antigen antibody
- ATP dependent DNA helicase 2 subunit 2 antibody
- ATP dependent DNA helicase II 80 kDa subunit antibody
see all
Images
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All lanes : Anti-Ku80 antibody [EPR3468] (ab80592) at 1/3000 dilution
Lane 1 : PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa -
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 ab80592.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab80592 (purified) at 1/50 dilution (20 µg/mL) immunoprecipitating Ku80 in HeLa whole cell lysate.
Lane 1 (input): HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab80592 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab80592 in HeLa whole cell lysate
For western blotting, ab80592 at 1/500 dilution (1.86 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
All lanes : Anti-Ku80 antibody [EPR3468] (ab80592) at 1/50000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa -
Immunofluorescence analysis of HeLa cells with 1/500 ab80592.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Ku80 with unpurified ab80592 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab80592 has been referenced in 39 publications.
- Li KV et al. A Surgical Kit for Stem Cell-Derived Retinal Pigment Epithelium Transplants: Collection, Transportation, and Subretinal Delivery. Front Cell Dev Biol 10:813538 (2022). PubMed: 35252183
- Stanaszek L et al. Transplantation of Human Glial Progenitors to Immunodeficient Neonatal Mice with Amyotrophic Lateral Sclerosis (SOD1/rag2). Antioxidants (Basel) 11:N/A (2022). PubMed: 35739947
- Takamiya S et al. Intravenous transplantation of amnion-derived mesenchymal stem cells promotes functional recovery and alleviates intestinal dysfunction after spinal cord injury. PLoS One 17:e0270606 (2022). PubMed: 35802703
- Pe'er-Nissan H et al. Placenta-Derived Mesenchymal-like Adherent Stromal Cells as an Effective Cell Therapy for Cocaine Addiction in a Rat Model. Pharmaceutics 14:N/A (2022). PubMed: 35890207
- Bradbury A et al. The Role of ATR Inhibitors in Ovarian Cancer: Investigating Predictive Biomarkers of Response. Cells 11:N/A (2022). PubMed: 35954206