Recombinant Anti-LEF1 antibody [EPR2029Y] (ab137872)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2029Y] to LEF1
- Suitable for: Flow Cyt, IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LEF1 antibody [EPR2029Y]
See all LEF1 primary antibodies -
Description
Rabbit monoclonal [EPR2029Y] to LEF1 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanIHC-P MouseRatHumanIP RatHumanWB RatHuman -
Immunogen
Synthetic peptide within Human LEF1 aa 100-200. The exact sequence is proprietary.
Database link: Q9UJU2 -
Positive control
- WB: Jurkat whole cell lysate (ab7899); Rat thymus tissue lysate; Human fetal lysate; His-tagged mouse LEF-1 recombinant protein (aa1-397). IHC-P: Human tonsil and thymus tissues; Mouse and rat spleen tissues. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2029Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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ICC/IF |
Human
|
IHC-P |
Mouse
Rat
Human
|
IP |
Rat
Human
|
WB |
Rat
Human
|
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
|
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WB |
1/1000. Predicted molecular weight: 44 kDa.
We don’t recommend this antibody for mouse in Western Blot. In our hands an extra band was observed in mouse tissue lysates. |
|
IHC-P | (2) |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
ICC/IF |
1/500.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 44 kDa. We don’t recommend this antibody for mouse in Western Blot. In our hands an extra band was observed in mouse tissue lysates. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
Target
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Function
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. -
Tissue specificity
Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. -
Sequence similarities
Belongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain. -
Domain
Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. -
Cellular localization
Nucleus. Found in nuclear bodies upon PIASG binding. - Information by UniProt
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Database links
- Entrez Gene: 51176 Human
- Entrez Gene: 16842 Mouse
- Entrez Gene: 161452 Rat
- Omim: 153245 Human
- SwissProt: Q9UJU2 Human
- SwissProt: P27782 Mouse
- SwissProt: Q9QXN1 Rat
- Unigene: 726506 Human
see all -
Alternative names
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
see all
Images
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Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/1000 dilution (purified) + Human fetal thymus lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)
Immunohistochemical staining of paraffin embedded human tonsil with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of Jurkat (Human T cell leukemia cell line from peripheral blood) cells with purified ab137872 at a working dilution of 1 in 500, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.
The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab137872 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
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Flow cytometric analysis of Jurkat cell line (human T cell leukemia T lymphocyte) fixed with 4% paraformaldehyde and permeabilized with 90% methanol labeling LEF1 with ab137872 at 1/600 dilution (red). This is compared with a Rabbit monoclonal IgG (ab172730) - Isotype control (black) and a unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody.
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Lane 1 (input): Jurkat (human T cell leukemia T lymphocyte) whole cell lysate, 10 μg
Lane 2 (+): Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137872 in Jurkat whole cell lysateab137872 immunoprecipitating LEF1 in Jurkat whole cell lysate. For western blotting, primary antibody used was ab137872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)
Immunohistochemical staining of paraffin embedded rat spleen with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)
Immunohistochemical staining of paraffin-embedded human thymus with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)
ab137872 staining LEF1 in paraffin embedded mouse spleen tissue by Immunohistochemistry. Antigen retrieval was by heat mediation using ab93684 (Tris/EDTA buffer, ph 9). Samples were incubated with primary antibody at 1/2000 dilution. A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on T cells in periarterial lymphatic sheath of mouse spleen is observed (PMID: 21685909).
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Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/2000 dilution (purified) + Rat thymus tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST
-
Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/10000 dilution (purified) + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST
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Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/1000 dilution + His-tagged mouse LEF-1 recombinant protein (aa1-397) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 1 secondBlocking and diluting buffer: 5% NFDM/TBST
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Lane 1 (input): Rat thymus lysate, 10μg
Lane 2 (+): Rat thymus lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137872 in rat thymus lysateab137872 immunoprecipitating LEF1 in rat thymus lysate. For western blotting, primary antibody used was ab137872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: 3 minutes
Protocols
References (28)
ab137872 has been referenced in 28 publications.
- Leng S et al. ß-Catenin and FGFR2 regulate postnatal rosette-based adrenocortical morphogenesis. Nat Commun 11:1680 (2020). PubMed: 32245949
- Wang X et al. MiR-34a-5p Inhibits Proliferation, Migration, Invasion and Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma by Targeting LEF1 and Inactivation of the Hippo-YAP1/TAZ Signaling Pathway. J Cancer 11:3072-3081 (2020). PubMed: 32226522
- Liu Y et al. Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma. Cell Rep 30:771-782.e6 (2020). PubMed: 31968252
- Pignatti E et al. Beta-Catenin Causes Adrenal Hyperplasia by Blocking Zonal Transdifferentiation. Cell Rep 31:107524 (2020). PubMed: 32320669
- Zhao Y et al. The transcription factor LEF1 promotes tumorigenicity and activates the TGF-ß signaling pathway in esophageal squamous cell carcinoma. J Exp Clin Cancer Res 38:304 (2019). PubMed: 31296250