Anti-LOK antibody (ab70484)
Key features and details
- Rabbit polyclonal to LOK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LOK antibody
See all LOK primary antibodies -
Description
Rabbit polyclonal to LOK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region between C terminal amino acids 900-968 of Human LOK
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Positive control
- Whole cell lysate from HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/15000. Predicted molecular weight: 112 kDa.
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IP |
Use at 1-4 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/15000. Predicted molecular weight: 112 kDa. |
IP
Use at 1-4 µg/mg of lysate. |
Target
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Function
Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues. -
Tissue specificity
Expressed predominantly in lymphoid organs. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 6793 Human
- Entrez Gene: 20868 Mouse
- Omim: 603919 Human
- SwissProt: O94804 Human
- SwissProt: O55098 Mouse
- Unigene: 519756 Human
- Unigene: 8235 Mouse
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Alternative names
- LOK antibody
- Lymphocyte oriented kinase antibody
- Lymphocyte-oriented kinase antibody
see all
Images
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All lanes : Anti-LOK antibody (ab70484) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 112 kDa
Chemiluminescence with exposure times of 1 to 2 minutes. -
Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70484 at 1 µg/mg lysate for IP.
Lane 1: input of Human LOK at 50µg
Lane 2: Other anti-human LOK antibody
Lane 3: Other anti-human LOK antibody plus blocking peptide
Lane 4: ab70484
Lane 5: ab70484 plus blocking peptide
Subsequent Western blot LOK detection was performed with 0.1 µg/mg ab70484.
Chemiluminescence with exposure times of 1 to 2 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab70484 has been referenced in 2 publications.
- Zhang L et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
- Zhang L et al. Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res 9:7079-7090 (2020). PubMed: 35117313