Overview

  • Product name

  • Description

    Rabbit polyclonal to LPA-4
  • Host species

    Rabbit
  • Specificity

    ab183076 detects endogenous level of total LPA-4 protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LPA-4 aa 358-370. The exact sequence is proprietary. Genbank: NP_005287
    Sequence:

    TNNGGELMLESTF


    Database link: Q99677

  • Positive control

    • Human leiomyosarcoma and liver cancer tissue lysates. Human esophagus cancer tissue.
  • General notes

    Previously labelled as P2Y9. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/450 - 1/2000. Predicted molecular weight: 42 kDa.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA.
  • Tissue specificity

    High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5730485F04Rik antibody
    • G protein coupled receptor 23 antibody
    • G-protein coupled receptor 23 antibody
    • GPR23 antibody
    • LPA receptor 4 antibody
    • LPA-4 antibody
    • LPA4 antibody
    • Lpar4 antibody
    • LPAR4_HUMAN antibody
    • Lysophosphatidic acid receptor 4 antibody
    • Lysophosphatidic Acid Receptor P2Y9 antibody
    • P2RY9 antibody
    • P2Y purinoceptor 9 antibody
    • P2Y5 like antibody
    • P2Y5-like receptor antibody
    • P2Y9 antibody
    • Purinergic receptor 9 antibody
    • Purinergic receptor, P2Y, G-protein coupled, 9 antibody
    see all

Images

  • All lanes : Anti-LPA-4 antibody (ab183076) at 1/450 dilution

    Lane 1 : Human leiomyosarcoma tissue lysate
    Lane 2 : Human liver cancer tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa


    Exposure time: 30 seconds


    10%SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling LPA-4 with ab183076 at a 1/30 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)

References

ab183076 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183076.
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