Product nameAnti-LPA-4 antibody
See all LPA-4 primary antibodies
DescriptionRabbit polyclonal to LPA-4
Specificityab183076 detects endogenous level of total LPA-4 protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human leiomyosarcoma and liver cancer tissue lysates. Human esophagus cancer tissue.
Previously labelled as P2Y9.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/450 - 1/2000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionReceptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA.
Tissue specificityHigh expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- 5730485F04Rik antibody
- G protein coupled receptor 23 antibody
- G-protein coupled receptor 23 antibody
All lanes : Anti-LPA-4 antibody (ab183076) at 1/450 dilution
Lane 1 : Human leiomyosarcoma tissue lysate
Lane 2 : Human liver cancer tissue lysate
Lysates/proteins at 40 µg per lane.
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa
Exposure time: 30 seconds
Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling LPA-4 with ab183076 at a 1/30 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)
ab183076 has not yet been referenced specifically in any publications.