Product nameAnti-LRDD antibody
See all LRDD primary antibodies
DescriptionRabbit polyclonal to LRDD
SpecificityBased on the immunogen information this product will recognise isoforms 1, 2, 3 and 4 of human LRDD.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey
- This antibody gave a positive signal in human liver tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab78389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 99.7 kDa (predicted molecular weight: 99.7 kDa).|
RelevanceThe death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B.
Cellular localizationCytoplasm. Nucleus.
- DKFZp434D229 antibody
- Leucine rich repeats and death domain containing antibody
- Leucine-rich repeats and death domain containing antibody
Anti-LRDD antibody (ab78389) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99.7 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa (possible non-specific binding), 37 kDa (possible non-specific binding), 66 kDa (possible isoform)
Exposure time: 3 minutes
LRDD has seven different isoforms (SwissProt) which all have differing molecular weights. The image shows the primary target (Isoform 1) at 99 kDa as well as isoform 4 (66 kDa).
ICC/IF image of ab78389 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78389, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HEK293, HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HepG2, and MCF-7 cells at 1µg/ml.
This product has been referenced in:
- Yao TH et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). Read more (PubMed: 29767244) »
- Klinck R et al. Alternative splicing in osteoclasts and Paget's disease of bone. BMC Med Genet 15:98 (2014). WB, ICC/IF ; Human . Read more (PubMed: 25115182) »