Recombinant Anti-LSM12 antibody [EPR12282-87] (ab173292)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12282-87] to LSM12
- Suitable for: ICC/IF, IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LSM12 antibody [EPR12282-87]
See all LSM12 primary antibodies -
Description
Rabbit monoclonal [EPR12282-87] to LSM12 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human LSM12. The exact sequence is proprietary.
Database link: Q3MHD2 -
Positive control
- 293T, HepG2, HeLa and fetal brain lysates; Human endometrial carcinoma tissue; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12282-87 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
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Notes |
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ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
WB
1/10000 - 1/50000. Predicted molecular weight: 22 kDa. |
Target
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Relevance
LSM12 belongs to the LSM12 family. The exact function of LSM12 is not known. -
Database links
- Entrez Gene: 124801 Human
- Entrez Gene: 268490 Mouse
- Entrez Gene: 287731 Rat
- Omim: 611793 Human
- SwissProt: Q3MHD2 Human
- SwissProt: Q9D0R8 Mouse
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Alternative names
- FLJ30656 antibody
- LSM12 homolog antibody
- LSM12 homolog (S. cerevisiae) antibody
see all
Images
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All lanes : Anti-LSM12 antibody [EPR12282-87] (ab173292) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LSM12 with ab173292 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173292 at 1/100 dilution.
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Western blot analysis on immunoprecipitation pellet from 1) HeLa cell lysate or 2) 1X PBS using ab173292 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab173292 has been referenced in 1 publication.
- Ayache J et al. P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Mol Biol Cell 26:2579-95 (2015). WB ; Human . PubMed: 25995375