Anti-Leptin Receptor antibody (ab50424)
Key features and details
- Goat polyclonal to Leptin Receptor
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Leptin Receptor antibody
See all Leptin Receptor primary antibodies -
Description
Goat polyclonal to Leptin Receptor -
Host species
Goat -
Specificity
This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1) of Leptin Receptor. -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog -
Immunogen
Synthetic peptide corresponding to Human Leptin Receptor aa 829-841 (internal sequence).
Sequence:C-TQDDIEKHQSDAG
Database link: P48357 -
Positive control
- WB: Human Cerebellum, Mouse Foetal Brain and Rat Brain lysate. Flow Cyt: K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway. -
Tissue specificity
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain. -
Domain
The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsOn ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 450207 Dog
- Entrez Gene: 3953 Human
- Entrez Gene: 16847 Mouse
- Entrez Gene: 24536 Rat
- Omim: 601007 Human
- SwissProt: P48357 Human
- SwissProt: P48356 Mouse
- SwissProt: Q62959 Rat
see all -
Alternative names
- CD 295 antibody
- CD295 antibody
- CD295 antigen antibody
see all
Images
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Lane 1 : Anti-Leptin Receptor antibody (ab50424) at 1.75 µg/ml
Lanes 2-3 : Anti-Leptin Receptor antibody (ab50424) at 1.5 µg/ml
Lane 1 : Human Cerebellum tissue lysate
Lane 2 : Mouse Foetal Brain tissue lysate
Lane 3 : Rat Brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 132 kDaLysate in RIPA buffer. Detected by chemiluminescence.
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Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab50424 has been referenced in 1 publication.
- Maranesi M et al. Leptin System in Obese Dog Skin: A Pilot Study. Animals (Basel) 10:N/A (2020). PubMed: 33316917