Anti-Leptin Receptor antibody (ab5593)
Key features and details
- Rabbit polyclonal to Leptin Receptor
- Suitable for: IHC-Fr, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Leptin Receptor antibody
See all Leptin Receptor primary antibodies -
Description
Rabbit polyclonal to Leptin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat Leptin Receptor aa 577-594 (extracellular).
Sequence:KEIQWKTHEVFDAKSKSA
(Peptide available asab5837) -
Positive control
- WB: Mouse lung tissue extract.
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General notes
As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
No concentration is available. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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WB | (4) |
1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837).
ab5593 detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R.
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IP |
Use at an assay dependent concentration. PubMed: 21151569
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
WB
1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837). ab5593 detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R.
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IP
Use at an assay dependent concentration. PubMed: 21151569 |
Target
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Function
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway. -
Tissue specificity
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain. -
Domain
The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsOn ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3953 Human
- Entrez Gene: 16847 Mouse
- Entrez Gene: 24536 Rat
- Omim: 601007 Human
- SwissProt: P48357 Human
- SwissProt: P48356 Mouse
- SwissProt: Q62959 Rat
- Unigene: 23581 Human
see all -
Alternative names
- CD 295 antibody
- CD295 antibody
- CD295 antigen antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab5593 has been referenced in 33 publications.
- Balatskiy A et al. Immature Vascular Smooth Muscle Cells in Healthy Murine Arteries and Atherosclerotic Plaques: Localization and Activity. Int J Mol Sci 23:N/A (2022). PubMed: 35163667
- Kim J & Lee M RMR-Related DNAJC6 Expression Suppresses Adipogenesis in 3T3-L1 Cells. Cells 11:N/A (2022). PubMed: 35456010
- Ramírez-Plascencia OD et al. Leptin and adiponectin regulate the activity of nuclei involved in sleep-wake cycle in male rats. Front Neurosci 16:907508 (2022). PubMed: 35937866
- Roy IM et al. Inhibition of SRC-mediated integrin signaling in bone marrow niche enhances hematopoietic stem cell function. iScience 25:105171 (2022). PubMed: 36204266
- Pan J et al. Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway. Mol Med 28:159 (2022). PubMed: 36539694