Anti-Leptin antibody (ab3583)
Key features and details
- Rabbit polyclonal to Leptin
- Suitable for: ICC, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Sheep, Human
- Isotype: IgG
Overview
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Product name
Anti-Leptin antibody
See all Leptin primary antibodies -
Description
Rabbit polyclonal to Leptin -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Sheep, Human
Predicted to work with: Goat, Horse, Chicken, Cow, Cat, Dog, Turkey, Chimpanzee, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Mouse Leptin aa 25-44.
Sequence:QKVQDDTKTLIKTIVTRIND
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Positive control
- ICC: 3T3-L1, HeLa and mesenchymal stem cells. IHC-P: Sheep brain tissue. Flow Cyt: 3T3-L1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 5 µg/ml.
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IHC-Fr | (1) |
1/50 - 1/200.
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Flow Cyt |
Use 3-5µl for 106 cells.
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Notes |
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ICC
Use a concentration of 5 µg/ml. |
IHC-Fr
1/50 - 1/200. |
Flow Cyt
Use 3-5µl for 106 cells. |
Target
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 493838 Cat
- Entrez Gene: 373955 Chicken
- Entrez Gene: 449638 Chimpanzee
- Entrez Gene: 280836 Cow
- Entrez Gene: 3952 Human
- Entrez Gene: 16846 Mouse
- Entrez Gene: 698728 Rhesus monkey
- Omim: 164160 Human
see all -
Alternative names
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
Images
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ab3583 staining Leptin in 3T3-L1 cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/2000) (Panel a). Nuclei were stained with DAPI (Panel b). F-actin stained with Rhodamine Phalloidin (panel c). Merged images shown in panel d.
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Immunohistochemical staining of Leptin in Sheep brain using ab3583.
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ICC image of ab3583 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3583, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ab3583 staining Leptin in Human mesenchyml stem cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% horse serum) for 1 hour at 22°C. A FITC-conjugated Mouse anti-rabbit IgG1 monoclonal was used as the secondary antibody (1/100).
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ab3583 staining Leptin in 3T3-L1 cells by Flow Cytometry. The sample was incubated with the primary antibody (3ug/ml in 2.5% BSA) for 2 hours at room temperature. An Alexa Fluor 488®-conjugated Goat anti-rabbit (1/400) was used as the secondary antibody. Red histogram represents ab3583, pink histogram represents isotype control, purple histogram represents unstained control, green histogram represents no primary antibody control
Protocols
Datasheets and documents
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Datasheet download
References (33)
ab3583 has been referenced in 33 publications.
- Zhao S et al. Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization. Anal Cell Pathol (Amst) 2022:9994906 (2022). PubMed: 35111566
- Luo SD et al. Aberrant miR-874-3p/leptin/EGFR/c-Myc signaling contributes to nasopharyngeal carcinoma pathogenesis. J Exp Clin Cancer Res 41:215 (2022). PubMed: 35778755
- Gao Y et al. Presence of leptin and its receptor in the ram reproductive system and in vitro effect of leptin on sperm quality. PeerJ 10:e13982 (2022). PubMed: 36187750
- Merigo F et al. Immunolocalization of leptin and leptin receptor in colorectal mucosa of ulcerative colitis, Crohn's disease and control subjects with no inflammatory bowel disease. Cell Tissue Res 383:1103-1122 (2021). PubMed: 33159578
- Gameiro A et al. Serum and Tissue Expression Levels of Leptin and Leptin Receptor Are Putative Markers of Specific Feline Mammary Carcinoma Subtypes. Front Vet Sci 8:625147 (2021). PubMed: 33644151