Overview

  • Product name
    Anti-Leu Enkephalin antibody
    See all Leu Enkephalin primary antibodies
  • Description
    Rabbit polyclonal to Leu Enkephalin
  • Host species
    Rabbit
  • Specificity
    Reacts with a wide range of other species. Leucine enkephalin, partial reactivity with methionine enkephalin, no detected reactivity with Substance P, VIP and CGRP.
  • Tested applications
    Suitable for: IHC-R, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH by a Glutaraldehyde linker.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituent: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-R
IHC-Fr
IHC-P
  • Application notes
    IHC-Fr: 1/500 - 1/4000.
    IHC-P: 1/500 - 1/4000.
    IHC-R: 1/500 - 1/4000.

    Using overnight incubation and ABC/PAP procedure. Dilutions up to 1/10,000 have been achieved under optimized conditions. 1/200 to 1/4000, using overnight incubation and indirect immunofluorescence procedure. All standard crosslinking agents may be used, but formaldehyde based solutions are recommended.
    Frozen sections and Vibratome slices are recommended for tract tracing.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • PENK antibody
      • Proenkephalin A antibody
      • Proenkephalin antibody

    Images

    • ab22619 (4µg/ml) staining leu enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    References

    ab22619 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thanks for your call today. I ran a BLAST with the immunogen of ab98128 against the Met and Leu sequences on SwissProt, and though the immunogen contains the entire Met sequence (YGGFM), it does not contain the full Leu sequence (only YGGF). So, this antibody might not react as strongly with the Leu enkephalin. Since none of our enkephalin antibodies have been tested in perfusion-fixed monkey tissue, I can offer a discount off a future purchase if you buy one of these antibodies now, test it in IHC-FoFr with monkey tissue, and submit feedback to us in the form of an Abreview. The discount is worth 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test one of these antibodies. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering. 2. Purchase the antibody either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-FoFr on monkey tissue. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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