Product nameAnti-Leu Enkephalin antibody
See all Leu Enkephalin primary antibodies
DescriptionRabbit polyclonal to Leu Enkephalin
SpecificityReacts with a wide range of other species. Leucine enkephalin, partial reactivity with methionine enkephalin, no detected reactivity with Substance P, VIP and CGRP.
Tested applicationsSuitable for: IHC-R, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide conjugated to KLH by a Glutaraldehyde linker.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab22619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/500 - 1/4000.
IHC-R: 1/500 - 1/4000.
Using overnight incubation and ABC/PAP procedure. Dilutions up to 1/10,000 have been achieved under optimized conditions. 1/200 to 1/4000, using overnight incubation and indirect immunofluorescence procedure. All standard crosslinking agents may be used, but formaldehyde based solutions are recommended.
Frozen sections and Vibratome slices are recommended for tract tracing.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceEnkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.
- PENK antibody
- Proenkephalin A antibody
- Proenkephalin antibody
ab22619 (4µg/ml) staining leu enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab22619 has not yet been referenced specifically in any publications.