Overview

  • Product name
    Anti-LexA DNA Binding Region antibody
    See all LexA DNA Binding Region primary antibodies
  • Description
    Rabbit polyclonal to LexA DNA Binding Region
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ChIP, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.
    Database link: POA7C2

  • Positive control
    • E. coli whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab174384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 22 kDa.
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
  • Cellular localization
    Nuclear
  • Alternative names
    • ExrA antibody
    • Lex A antibody
    • LexA antibody
    • LexA repressor antibody
    • Spr antibody
    • Tsl antibody
    • UmuA antibody
    see all

Images

  • All lanes : Anti-LexA DNA Binding Region antibody (ab174384) at 1/1000 dilution

    Lane 1 : Marker
    Lane 2 : E. coli whole cell lysate

    Predicted band size: 22 kDa

References

This product has been referenced in:
  • Hishida T  et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004). WB ; Escherichia coli . Read more (PubMed: 15289460) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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