Anti-Lin28A antibody (ab46020)
Key features and details
- Rabbit polyclonal to Lin28A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lin28A antibody
See all Lin28A primary antibodies -
Description
Rabbit polyclonal to Lin28A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Lin28A.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (6) |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).
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ICC/IF | (3) |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. -
Tissue specificity
Expressed in embryonic stem cells (ES cells), placenta and testis. -
Sequence similarities
Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain. -
Developmental stage
Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. -
Domain
The CSD domain is required for function in muscle differentiation. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. - Information by UniProt
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Database links
- Entrez Gene: 79727 Human
- Omim: 611043 Human
- SwissProt: Q9H9Z2 Human
- Unigene: 86154 Human
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Alternative names
- CSDD1 antibody
- FLJ12457 antibody
- LIN 28 antibody
see all
Images
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All lanes : Anti-Lin28A antibody (ab46020) at 1/1000 dilution
Lane 1 : PC-3 cell extract in lysis buffer containing NP-40 at 45 µg
Lane 2 : PC-3 cell extract (Trizol bottom phase) at 30 µg
Lane 3 : Tera-2 cell extract (Trizol bottom phase) at 30 µg
Lane 4 : Tera-2 cell extract in lysis buffer containing NP-40 at 45 µg
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/4000 dilution
Predicted band size: 23 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.It is unclear why lin28A runs at a larger size than its predicted moleular weight but recombinant lin28 protein is also known to run at around 30 kDa.
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Human Embryonic stem cells were fixed in PFA and then incubated with the primary antibody ab46020 (1:1000) diluted in 2% BSA for 16 hours at 4C. Cells where then washed and incubated with a Cy3 conjugated goat anti-rabbit polyclonal antibody (1:400). Cells where then washed and visualised.
Protocols
Datasheets and documents
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Datasheet download
References (93)
ab46020 has been referenced in 93 publications.
- Miklas JW et al. Amino acid primed mTOR activity is essential for heart regeneration. iScience 25:103574 (2022). PubMed: 34988408
- Lecante LL et al. Acetaminophen (APAP, Paracetamol) Interferes With the First Trimester Human Fetal Ovary Development in an Ex Vivo Model. J Clin Endocrinol Metab 107:1647-1661 (2022). PubMed: 35147701
- Yang P et al. T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia. PeerJ 9:e11545 (2021). PubMed: 34141486
- Osborne JK et al. Lin28 paralogs regulate lung branching morphogenesis. Cell Rep 36:109408 (2021). PubMed: 34289374
- Luo C et al. LINC01094 promotes pancreatic cancer progression by sponging miR-577 to regulate LIN28B expression and the PI3K/AKT pathway. Mol Ther Nucleic Acids 26:523-535 (2021). PubMed: 34631282