Anti-Lysosomal acid lipase/LAL antibody (ab154356)
Key features and details
- Rabbit polyclonal to Lysosomal acid lipase/LAL
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Lysosomal acid lipase/LAL antibody
See all Lysosomal acid lipase/LAL primary antibodies -
Description
Rabbit polyclonal to Lysosomal acid lipase/LAL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Lysosomal acid lipase/LAL aa 164-399.
-
Positive control
- A549 whole cell lysate, H1299 whole cell lysate, mouse liver lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
Target
-
Function
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. -
Involvement in disease
Defects in LIPA are the cause of Wolman disease (WOD) [MIM:278000]. WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year.
Defects in LIPA are the cause of cholesteryl ester storage disease (CESD) [MIM:278000]. CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family. -
Cellular localization
Lysosome. - Information by UniProt
-
Database links
- Entrez Gene: 3988 Human
- Entrez Gene: 16889 Mouse
- Omim: 278000 Human
- SwissProt: P38571 Human
- SwissProt: Q9Z0M5 Mouse
- Unigene: 643030 Human
- Unigene: 157545 Mouse
-
Alternative names
- Acid cholesteryl ester hydrolase antibody
- CESD antibody
- cholesterol ester hydrolase antibody
see all
Images
-
Anti-Lysosomal acid lipase/LAL antibody (ab154356) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
All lanes : Anti-Lysosomal acid lipase/LAL antibody (ab154356) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab154356 has been referenced in 4 publications.
- Carotti S et al. Molecular and histological traits of reduced lysosomal acid lipase activity in the fatty liver. Cell Death Dis 12:1092 (2021). PubMed: 34795230
- Wang H et al. An AMPK-dependent, non-canonical p53 pathway plays a key role in adipocyte metabolic reprogramming. Elife 9:N/A (2020). PubMed: 33320092
- Wang C et al. Autophagic lipid metabolism sustains mTORC1 activity in TSC-deficient neural stem cells. Nat Metab 1:1127-1140 (2019). PubMed: 32577608
- Llewellyn KJ et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB ; Mouse . PubMed: 26134519