Anti-Lysosomal acid lipase/LAL antibody (ab89771)
Key features and details
- Mouse polyclonal to Lysosomal acid lipase/LAL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lysosomal acid lipase/LAL antibody
See all Lysosomal acid lipase/LAL primary antibodies -
Description
Mouse polyclonal to Lysosomal acid lipase/LAL -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Lysosomal acid lipase/LAL. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH12287.1)
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Positive control
- Human pancreas tissue lysate, Lysosomal acid lipase/LAL transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. |
Target
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Function
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. -
Involvement in disease
Defects in LIPA are the cause of Wolman disease (WOD) [MIM:278000]. WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year.
Defects in LIPA are the cause of cholesteryl ester storage disease (CESD) [MIM:278000]. CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 3988 Human
- Omim: 278000 Human
- SwissProt: P38571 Human
- Unigene: 643030 Human
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Alternative names
- Acid cholesteryl ester hydrolase antibody
- CESD antibody
- cholesterol ester hydrolase antibody
see all
Images
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Anti-Lysosomal acid lipase/LAL antibody (ab89771) at 1 µg/ml + human pancreas tissue lysate at 50 µg
Predicted band size: 45 kDa
Observed band size: 45 kDa -
All lanes : Anti-Lysosomal acid lipase/LAL antibody (ab89771) at 1 µg/ml
Lane 1 : Lysosomal acid lipase/LAL transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Datasheets and documents
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Datasheet download
References (0)
ab89771 has not yet been referenced specifically in any publications.