Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)
Key features and details
- Mouse monoclonal [3C7] to MAG/GMA - BSA and Azide free
- Suitable for: ICC, Flow Cyt, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-MAG/GMA antibody [3C7] - BSA and Azide free
See all MAG/GMA primary antibodies -
Description
Mouse monoclonal [3C7] to MAG/GMA - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human MAG/GMA aa 100-250.
Database link: P20916 -
Positive control
- Partial tagged recombinant Human MAG/GMA protein (Immunogen), Jurkat whole cell lysate (ab7899). This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH.
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General notes
This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3C7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
Isoform a, 69 kDa; Isoform b, 63 kDa. |
Notes |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. Isoform a, 69 kDa; Isoform b, 63 kDa. |
Target
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Function
Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4099 Human
- Omim: 159460 Human
- SwissProt: P20916 Human
- Unigene: 643440 Human
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Alternative names
- GMA antibody
- MAG antibody
- MAG_HUMAN antibody
see all
Images
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Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780) at 5 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 63 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
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ab89780 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89780 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780) at 5 µg/ml + Partial tagged recombinant Human MAG/GMA protein at 0.2 µg
Predicted band size: 63 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (54)
ab89780 has been referenced in 54 publications.
- Liu J et al. Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury. Neural Regen Res 17:673-681 (2022). PubMed: 34380909
- Wan T et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nat Commun 13:1134 (2022). PubMed: 35241660
- Sánchez-de la Torre A et al. Cannabinoid CB1 receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice. Cell Death Dis 13:585 (2022). PubMed: 35798697
- Zhao Y et al. Vascular endothelium deploys caveolin-1 to regulate oligodendrogenesis after chronic cerebral ischemia in mice. Nat Commun 13:6813 (2022). PubMed: 36357389
- Li X et al. The Role and Mechanism of AMIGO3 in the Formation of Aberrant Neural Circuits After Status Convulsion in Immature Mice. Front Mol Neurosci 14:748115 (2021). PubMed: 34650403