Anti-MARCKS antibody (ab254051)
Key features and details
- Chicken polyclonal to MARCKS
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-MARCKS antibody
See all MARCKS primary antibodies -
Description
Chicken polyclonal to MARCKS -
Host species
Chicken -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Recombinant full length protein corresponding to Human MARCKS. Produced/ purified from E. coli.
Database link: P29966 -
Positive control
- WB: HeLa cell lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/1000.
|
|
WB |
1/10000.
|
Notes |
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ICC/IF
1/1000. |
WB
1/10000. |
Target
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Function
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. -
Sequence similarities
Belongs to the MARCKS family. -
Post-translational
modificationsPhosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. -
Cellular localization
Cytoplasm > cytoskeleton. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4082 Human
- Omim: 177061 Human
- SwissProt: P29966 Human
- Unigene: 519909 Human
- Unigene: 712721 Human
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Alternative names
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab254051 has not yet been referenced specifically in any publications.