Anti-MAX antibody (ab101271)
Key features and details
- Rabbit polyclonal to MAX
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MAX antibody
See all MAX primary antibodies -
Description
Rabbit polyclonal to MAX -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide within Human MAX aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P61244 -
Positive control
- HeLa and 293T whole cell lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human ovarian tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 18 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 18 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. -
Tissue specificity
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. -
Involvement in disease
Pheochromocytoma -
Sequence similarities
Belongs to the MAX family.
Contains 1 bHLH (basic helix-loop-helix) domain. -
Post-translational
modificationsReversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes. -
Cellular localization
Nucleus. Cell projection, dendrite. - Information by UniProt
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Database links
- Entrez Gene: 4149 Human
- Entrez Gene: 17187 Mouse
- Omim: 154950 Human
- SwissProt: P52162 Chicken
- SwissProt: P61244 Human
- SwissProt: P28574 Mouse
- Unigene: 285354 Human
- Unigene: 268548 Mouse
see all -
Alternative names
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MAX with ab101271 at 1/1000 (1µg/ml).
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All lanes : Anti-MAX antibody (ab101271) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3 minutes -
Detection of MAX in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101271 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents Immunoprecipitates from control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab101271 has been referenced in 4 publications.
- Yamashita T et al. Decreased MYC-associated factor X (MAX) expression is a new potential biomarker for adverse prognosis in anaplastic large cell lymphoma. Sci Rep 10:10391 (2020). PubMed: 32587329
- Chang X et al. A Novel Phenotype of Germline Pathogenic Variants in MAX: Concurrence of Pheochromocytoma and Ganglioneuroma in a Chinese Family and Literature Review. Front Endocrinol (Lausanne) 11:558 (2020). PubMed: 32973681
- Nicolson NG et al. Transcription Factor Profiling Identifies Spatially Heterogenous Mediators of Follicular Thyroid Cancer Invasion. Endocr Pathol 31:367-376 (2020). PubMed: 33063251
- Malchenko S et al. Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. PLoS One 12:e0173106 (2017). IHC-P ; Human . PubMed: 28249000