Anti-MAX antibody (ab9700)
Key features and details
- Goat polyclonal to MAX
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAX antibody
See all MAX primary antibodies -
Description
Goat polyclonal to MAX -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human MAX aa 100-200 (C terminal). (NP_660087.1)
Database link: NP_002373.3 -
Positive control
- WB: Jurkat cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. -
Tissue specificity
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. -
Involvement in disease
Pheochromocytoma -
Sequence similarities
Belongs to the MAX family.
Contains 1 bHLH (basic helix-loop-helix) domain. -
Post-translational
modificationsReversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes. -
Cellular localization
Nucleus. Cell projection, dendrite. - Information by UniProt
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Database links
- Entrez Gene: 4149 Human
- Entrez Gene: 17187 Mouse
- Entrez Gene: 60661 Rat
- Omim: 154950 Human
- SwissProt: P61244 Human
- SwissProt: P28574 Mouse
- SwissProt: P52164 Rat
- Unigene: 285354 Human
see all -
Alternative names
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
see all
Images
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Anti-MAX antibody (ab9700) at 0.01 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µg
Predicted band size: 18 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
Datasheets and documents
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Datasheet download
References (0)
ab9700 has not yet been referenced specifically in any publications.