Anti-MC4-R antibody (ab24233)
Key features and details
- Rabbit polyclonal to MC4-R
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MC4-R antibody
See all MC4-R primary antibodies -
Description
Rabbit polyclonal to MC4-R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse MC4-R aa 1-100 (N terminal).
Database link: P56450 -
General notes
This antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
This antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (6) |
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). A larger band is also detected at 55 kDa. This most likely represents the glycosylated form of the protein.
|
IHC-P | (1) |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). A larger band is also detected at 55 kDa. This most likely represents the glycosylated form of the protein. |
IHC-P
Use a concentration of 1 µg/ml. |
Target
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Function
Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase. -
Tissue specificity
Brain, placental, and gut tissues. -
Involvement in disease
Defects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4160 Human
- Entrez Gene: 25635 Rat
- Omim: 155541 Human
- SwissProt: P32245 Human
- SwissProt: P70596 Rat
- Unigene: 532833 Human
- Unigene: 10620 Rat
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Alternative names
- MC4 Receptor antibody
- MC4-R antibody
- MC4R antibody
see all
Images
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All lanes : Anti-MC4-R antibody (ab24233)
Lane 1 : Human cerebella cortex pellet.
Lane 2 : Human hippocampus homogenate.
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Additional bands at: 55 kDa (possible glycosylated form) -
Immunoperoxidase staining of rat brain section with ab24233. Note the tadpole like staining of cortex neurons.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab24233 has been referenced in 20 publications.
- Correia AS et al. Obesity-Related Genes Expression in Testes and Sperm Parameters Respond to GLP-1 and Caloric Restriction. Biomedicines 10:N/A (2022). PubMed: 36289871
- Yu S et al. Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats. J Neuroinflammation 18:26 (2021). PubMed: 33468172
- Pereira SC et al. Obesity-related genes are expressed in human Sertoli cells and modulated by energy homeostasis regulating hormones. J Cell Physiol 236:5265-5277 (2021). PubMed: 33368221
- Pho H et al. Leptin receptor expression in the dorsomedial hypothalamus stimulates breathing during NREM sleep in db/db mice. Sleep 44:N/A (2021). PubMed: 33624805
- Huang XM et al. Mutations in MC4R facilitate the angiogenic activity in patients with orbital venous malformation. Exp Biol Med (Maywood) 245:956-963 (2020). PubMed: 32363922