Anti-MIF antibody [2A10-4D3] (ab55445)
Key features and details
- Mouse monoclonal [2A10-4D3] to MIF
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-MIF antibody [2A10-4D3]
See all MIF primary antibodies -
Description
Mouse monoclonal [2A10-4D3] to MIF -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-116 of Human MIF
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Positive control
- IHC-P: Human lung cell carcinoma tissue. Flow cyt: Jurkat cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2A10-4D3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
Involvement in disease
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
Sequence similarities
Belongs to the MIF family. -
Cellular localization
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
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Database links
- Entrez Gene: 4282 Human
- Omim: 153620 Human
- SwissProt: P14174 Human
- Unigene: 407995 Human
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Alternative names
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
Images
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Western blot against tagged recombinant protein immunogen using ab55445 MIF antibody at 1μg/ml. Predicted band size of immunogen is 38 kDa
This image was generated using the ascites version of the product.
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MIF antibody (ab55445) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lung, adenosqumous cell carcinoma tissue.
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Overlay histogram showing Jurkat cells stained with ab55445 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55445, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product. -
Paraffin embedded human lung squamous cell carcinoma tissue stained for MIF using ab55445 at 6 µg/ml in immunohistochemical analysis.
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Immunohistochemical analysis of Human nasopharyngeal carcinoma, staining MIF with ab55445 at 1/100 dilution.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab55445 has been referenced in 15 publications.
- An HJ et al. Prognostic role of macrophage migration inhibitory factor in patients with clear cell renal cell carcinoma. Medicine (Baltimore) 99:e23277 (2020). PubMed: 33327252
- Koh HM et al. Prognostic role of macrophage migration inhibitory factor expression in patients with squamous cell carcinoma of the lung. Thorac Cancer 10:2209-2217 (2019). PubMed: 31602798
- Kang Y et al. Macrophage migration inhibitory factor is a novel prognostic marker for human oral squamous cell carcinoma. Pathol Res Pract 214:1192-1198 (2018). PubMed: 30041958
- Kassaar O et al. Macrophage Migration Inhibitory Factor is subjected to glucose modification and oxidation in Alzheimer's Disease. Sci Rep 7:42874 (2017). PubMed: 28230058
- Wang ZC et al. Expression of STING and MIF in tumor infiltration lymphocytes as prognostic factors in patients with ESCC. Int J Clin Exp Pathol 10:10066-10074 (2017). PubMed: 31966897